辛伐他汀对急性脑梗死患者血浆中PTEN、HIF-1α表达和预后的影响

The effects of simvastatin on the expressions of PTEN,HIF-1α and prognosis in patients with acute cerebral infarction

目的探究辛伐他汀对急性脑梗死患者血浆中10号染色体上缺失的磷酸酶与张力蛋白同源物基因(PTEN)、缺氧诱导因子-1α(HIF-1α)表达和预后的影响。方法选取延安大学附属医院于2017年4月至2019年4月收治的急性脑梗死患者80例作为研究对象,随机分为两组,辛伐他汀组和对照组各40例。对照组患者给予常规治疗,辛伐他汀组患者在常规治疗的基础上配合辛伐他汀治疗。分别应用实时荧光定量法(RT-qPCR)、酶联免疫吸附法(ELISA)检测患者治疗前后血浆中PTEN mRNA表达和HIF-1α蛋白水平,并对两组患者的预后状况进行评估。结果治疗后,两组患者血浆PTEN mRNA表达水平均显著升高(P<0.05),且治疗后辛伐他汀组血浆PTEN mRNA表达水平明显高于对照组(P<0.05);治疗后,两组患者血清HIF-1α蛋白水平、NIHSS评分均比治疗前显著降低(P<0.05);且治疗后辛伐他汀组血清HIF-1α蛋白水平、NIHSS评分明显低于对照组(P<0.05);治疗后,辛伐他汀组预后良好率87.50%,显著高于对照组67.50%(P<0.05)。结论辛伐他汀能明显降低急性脑梗死患者神经功能损伤程度,改善患者预后,疗效显著,值得临床推广使用。

Objective To investigate the effects of simvastatin on the expressions of phosphatase and tensin homology deleted on chromosome ten(PTEN),hypoxia inducible factor-1α(HIF-1α)and prognosis in patients with acute cerebral infarction.Methods 80 patients with acute cerebral infarction admitted in Affiliated Hospital of Yan′an University from April 2017 to April 2019 were selected as the subjects and randomly divided into two groups,with 40 in simvastatin group and 40 in control group.The patients in the control group were given routine treatment,and the patients in simvastatin group were treated with simvastatin on the basis of routine treatment.The levels of PTEN mRNA and HIF-1αprotein in plasma were detected by real time fluorescence quantitative method(RT-qPCR)and enzyme linked immunosorbent assay(ELISA)before and after treatment respectively,and the prognosis of the two groups was evaluated.Results After treatment,the expression of plasma PTEN mRNA in the two groups increased significantly(P<0.05),and the expression of plasma PTEN mRNA in simvastatin group was significantly higher than that in the control group(P<0.05);the protein levels of HIF-1αand NIHSS in the two groups were significantly lower than those before treatment(P<0.05);and the levels of HIF-1αand NIHSS in simvastatin group were significantly lower than those in the control group(P<0.05);after treatment,the good prognosis rate of simvastatin group was 87.50%,which was significantly higher than 67.50%of control group(P<0.05).Conclusion Simvastatin can significantly reduce the degree of neurological damage in patients with acute cerebral infarction,improve the prognosis of patients,and has a significant effect,which is worthy of clinical application.