DNA镊子型电致化学发光生物传感器高灵敏检测MicroRNA-21

DNA Tweezers Electrochemiluminescence Biosensor for Highly Sensitive Detection of MicroRNA-21

利用DNA的生物相容性,以DNA纳米技术为基础构建了一种新型的电致化学发光(ECL)生物传感器,实现了对microRNA-21的超灵敏检测。首先,我们通过合理的设计合成了两端分别标记有能量转移供受体的DNA镊子。通过DNA酶诱导的目标物循环得到双倍的引发链。将该引发链修饰在处于“打开状态”的DNA镊子中,触发了DNA镊子的“关闭状态”,从而能量供受体对距离更近,进而发生能量转移,增加了受体(量子点)的ECL强度。

On the basis of the DNA nanotechnology,a novel ECL biosensor for highly sensitive detection of miRNA-21 according to the favorable biocompatibility of DNA was developed.Firstly,a DNA tweezer with the energy donor(AF488)and acceptor(QDs)labeled on the two ends was synthesized.Furthermore,two folds of the reporter DNA can be obtained through DNA zyme-induced target recycling.The reporter DNA was introduced to the“opened”tweezer which triggered the“close”state of the nanotweezer,thus making the distance between AF488 and QDs and increasing ECL intensity of QDs significantly.