血管内皮生长因子、转录因子-21在宫颈癌中的表达及对放疗敏感性的影响

Expression of vascular endothelial growth factor and transcription factor 21 in cervical cancer and its effect on radiotherapy sensitivity

目的探讨血管内皮生长因子(VEGF)、转录因子-21(TCF-21)在宫颈癌中的表达及对放疗敏感性的影响。方法选取120例宫颈癌患者,均接受放疗,免疫组化法检测宫颈癌组织中VEGF、TCF-21的表达情况,酶联免疫吸附法检测血清肿瘤标志物[鳞状细胞癌相关抗原(SCC-Ag)、癌胚抗原(CEA)、糖类抗原72-4(CA72-4)]水平,比较不同VEGF、TCF-21表达情况宫颈癌患者放疗疗效及血清肿瘤标志物水平。结果120例宫颈癌患者宫颈癌组织中,VEGF阳性表达83例,阳性表达率为69.17%(83/120),TCF-21阳性表达34例,阳性表达率为28.33%(34/120)。VEGF阳性表达宫颈癌患者的放疗总有效率为63.86%,明显低于VEGF阴性表达患者的97.30%(P﹤0.01),血清SCC-Ag、CEA、CA72-4水平均明显高于VEGF阴性表达患者(P﹤0.01)。TCF-21阳性表达宫颈癌患者的放疗总有效率为94.12%,明显高于TCF-21阴性表达患者的60.47%(P﹤0.01),血清SCC-Ag、CEA、CA72-4水平均明显低于TCF-21阴性表达患者(P﹤0.01)。结论TCF-21能够增加放疗敏感性,促进肿瘤细胞凋亡;VEGF则会降低放疗敏感性,促进肿瘤细胞侵袭。

Objective To investigate the expression of vascular endothelial growth factor(VEGF)and transcription factor-21(TCF-21)in cervical cancer and its effect on radiotherapy sensitivity.Method A total of 120 patients with cer-vical cancer were selected,all received radiotherapy,and immunohistochemistry assay was used to detect the expression of VEGF and TCF 21 in cervical cancer tissue.Enzyme-linked immunosorbent assay to detect serum tumor markers[squamous cell carcinoma-related antigen(SCC-Ag),carcinoembryonic antigen(CEA),carbohydrate antigen 72-4(CA72-4)]levels,and compare the different expressions of VEGF and TCF-21 in cervical cancer patients with radiotherapy effi-cacy and serum tumor marker levels.Result Among 120 cervical cancer patients,83 cases were positive for VEGF,and the positive expression rate was 69.17%(83/120),TCF-21 was positive for 34 cases,and the positive expression rate was 28.33%(34/120).The effective rate of radiotherapy for patients with VEGF-positive cervical cancer was 63.86%,which was significantly lower than that of patients with VEGF-negative expression of 97.30%(P<0.01),the serum levels of SCC-Ag,CEA,and CA72-4 were significantly higher than those of VEGF-negative patients(P<0.01).The effective rate of radiotherapy for patients with TCF-21 positive expression of cervical cancer was 94.12%,which was significantly high-er than that of patients with TCF-21 negative expression of 60.47%(P<0.01),the serum levels of SCC-Ag,CEA,and CA72-4 were significantly lower than TCF-21 negative expression patients(P<0.01).Conclusion TCF-21 can increase the sensitivity of radiotherapy and promote tumor cell apoptosis;VEGF can reduce the sensitivity of radiotherapy and pro-mote tumor cell invasion.