槐花散白头翁汤合用对急性放射性直肠炎大鼠的治疗作用及其机制研究

Effects of Huaihuasan and baitouweng formular on rats with acute radiation proctitis and its mechanism

目的:观察槐花散合白头翁汤对大鼠急性放射性直肠炎(ARP)的疗效。方法:选用清洁级SD大鼠40只,随机分为空白组(Control)、模型组(Model)、美沙拉嗪组(Mesalazine)、槐花散合白头翁汤组(Formula),每组10只。除Control组外其他三组大鼠盆腔均接受6MV-X射线20 GY剂量照射,制作大鼠急性放射性直肠炎模型。大鼠成模后每天给予相应药物灌胃治疗,按成人临床等效剂量(体表面积法折算),Control组、Model组均给予生理盐水灌胃每天10 ml/kg,Mesalazine组给予美沙拉嗪溶液0.27 g/kg灌胃,Formular组分别给予(0.91 g/kg)灌胃,连续14 d。灌胃后第14日处死所有大鼠,评估大鼠一般情况,HE染色观察大鼠直肠组织的病理变化,酶联免疫吸附法(ELISA)检测细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)的含量,免疫印迹法(Western blot)检测组织中核转录因子-kappaB(NF-κB)的表达。结果:与Control组比较,Model组大鼠出现严重腹泻症状,直肠黏膜组织出血、溃烂,坏死严重,NF-κB、、ICAM-1、VCAM-1表达明显增高(P<0.01)。与Model组比较,Mesalazine组和Formular组明显改善大鼠临床症状和肠粘膜愈合,下调了直肠组织中NF-κB P65蛋白表达,血清中ICAM-1、VCAM-1的含量明显降低,差异有统计学意义(P<0.05)。结论:槐花散合白头翁汤可以通过降低NF-κB、ICAM-1、VCAM-1表达,改善ARP大鼠症状和直肠粘膜损伤。

Objective:To investigate the effects of huaihuasan and baitouweng formular on acute radiation proctitis(ARP)in rats.Methods:Forty clean grade SD rats were randomly divided into control group,model group,mesalazine group and formula group.Except the control group,all the other three groups received 6 MV-20 GY dose of X-ray irradiation in the pelvic cavity,and the rat model of acute radiation proctitis was established.The rats were given daily gavage intervention with the corresponding drugs after mold formation.According to the adult clinical equivalent dose(body surface area),the control group and the model group were given 10 ml/kg saline daily,the Mesalazine group was treated with Mesalazine solution at the dose of 0.27 g/kg,and the Formular group was given(0.91 g/kg)respectively for 14 days.All rats were killed on the 14th day after administration.To evaluate the general situation of the rats,HE staining was used to observe the pathological changes in the rectal tissues of the rats.The contents of intercellular adhesion molecule-1(ICAM-1)and vascular adhesion molecule-1(VCAM-1)were detected by Elisa,and the expression of NF-κb P65 in the tissues were detected by Western blot.Results:Compared with the model group,in the mesalazine group and the formular group,the clinical symptoms and intestinal mucosal healing of rats were improved significantly,the expression level of NF-κB P65 protein in the rectal tissues and the serum contents of ICAM-1 and VCAM-1 were decreased with statistically significant differences(P<0.05).Conclusion:Huaihuasan and baitouweng formular can reduce NF-κB,ICAM-1 and VCAM-1 to improve symptoms and rectal mucosal injury in ARP rats.