推拿对慢性应激大鼠抑郁行为的影响及其机制

Effect of massage on depressive behavior of chronic stress rats and its mechanism

目的:探讨推拿对慢性应激大鼠抑郁行为的影响及其作用机制。方法:制备慢性轻度不可预见性的应激大鼠模型[1-2],造模21 d后,进行推拿治疗14 d。分组:空白对照组、模型组、推拿组、氟西汀组,每组10只。每日推拿膀胱经重要穴位10 min(间隔2 min,共2次)。通过体质量检测、旷场、糖水消耗实验和水迷宫实验评价抑郁模型大鼠行为学改变情况;蛋白质免疫印迹法(Western blot)法检测大鼠海马及前额叶皮质组织中ERK/P-ERK、BDNF蛋白表达情况。结果:模型组与空白组比较,大鼠的体质量、旷场、糖水消耗实验和水迷宫数据均显著下降(P<0.01),P-ERK、BDNF蛋白含量均显著降低(P<0.01);推拿组和氟西汀组与模型组比较,大鼠的体质量、旷场实验、糖水消耗实验和水迷宫实验数据均显著上升(P<0.01),推拿组和氟西汀组大鼠海马及前额叶皮质组织中P-ERK、BDNF蛋白含量均显著升高(P<0.05,P<0.01),氟西汀组升高更为显著(P<0.01)。结论:推拿可上调大鼠海马及前额叶皮质组织中ERK蛋白的磷酸化水平,激活ERK信号通路,促进效应蛋白BDNF的表达,改善慢性应激大鼠的抑郁行为。

Objective:To investigate the effects and mechanisms of massage on the depressive behavior of rats with chronic stress.Methods:The rats were subjected to chronic unpredictable mild stress for 21 days and then treated with massage for 14 days.They were divided into the following groups:blank control group,model group,massage group and fluoxetine group,with 10 rats in each group.The important acupoints of bladder meridian were massaged for 10 minutes every day(with an interval of 2 minutes,2 times in total).Body weight,open field test,sucrose intake test and water maze test were used to evaluate the behavioral changes.The expressions of Erk/p-ERK(Extracellular signal-related kinases/Phosphorylation extracellular signal-related kinases)and BDNF in prefrontal cortex were detected by Western Blot.Results:The body weight,open field sucrose intake test and water maze data of the model group were significantly lower than those of the Control Group(P<0.01),and the contents of p-ERK and BDNF protein in prefrontal cortex were decreased significantly(P<0.01).The body weight,open field test,sucrose intake test and water maze test data of rats in massage group and fluoxetine group were significantly higher than those in model group(P<0.01).The contents of p-ERK and BDNF in frontal cortex were increased significantly(P<0.05,P<0.01),especially in fluoxetine group(P<0.01).Conclusion:Massage may increase the phosphorylation level of ERK protein in hippocampus and prefrontal cortex,activate ERK signaling pathway,promote the expression of BDNF,and improve the depression behavior of chronic stress rats.