马钱子苷对神经干细胞的增殖、存活和分化的调节作用

Regulatory effects of Loganin on the proliferation,survival,and differentiation of nueral stem cells

为探讨不同浓度的马钱子苷对神经干细胞的增殖、存活和分化的调节作用及其相关分子机制,本实验从成年小鼠大脑中分离培养了神经干细胞,用不同浓度的马钱子苷进行干预,观察马钱子苷对神经干细胞增殖、存活和分化的影响。结果显示:成年小鼠神经干细胞在含有中、高浓度马钱子苷的增殖培养基中培养5 d和7 d后,神经球数量和直径与对照相比显著增加(P<0.05);中、高剂量的马钱子苷能够促进神经干细胞的有丝分裂,低剂量马钱子苷处理显著促进神经干细胞发生分化(P<0.01),并增加神经元和星形胶质细胞的数量及比例(P<0.01);中、高剂量马钱子苷抑制神经干细胞分化(P<0.05),高剂量的马钱子苷使得神经元的数量减少(P<0.05)。研究结果表明,高浓度的马钱子苷能够促进神经干细胞存活,并通过促进神经干细胞有丝分裂来提高其增殖能力;低浓度的马钱子苷促进神经干细胞分化,有利于神经再生和少突胶质细胞再生。研究结果为神经干细胞治疗中枢神经系统疾病的研究奠定了理论和实验基础。

The present study aimed to investigate the regulatory effects of different concentrations of Loganin on the proliferation,survival,and differentiation of neural stem cells(NSCs)and its related molecular mechanisms.NSCs were isolated and cultured from the brain of adult mouse.The effects of Loganin on the proliferation,survival,and differentiation of NSCs were determined using different concentrations of Loganin.The results showed that the number and diameter of adult mouse NSCs significantly increased compared with those of control mouse after culturing in medium and high concentrations of Loganin for 5 days and 7 days(P<0.05).Medium and high concentrations of Loganin could promote the mitosis of NSCs.Low-dose Loganin treatment significantly promoted the differentiation of NSCs(P<0.01)and increased the number and proportion of neurons and astrocytes(P<0.01).However,medium and high concentrations of Loganin inhibited the differentiation of NSCs(P<0.05).Furthermore,high concentrations of Loganin reduced the number of neurons(P<0.05).These results suggest that a high concentration of Loganin could promote the survival of NSCs and enhance their proliferative ability by promoting the mitosis of NSCs.On the other hand,a low concentration of Loganin could promote the differentiation of NSCs and is beneficial for neurogenesis and oligodendrocyte regeneration.These results lay the theoretical and experimental foundation for research on NSCs for the treatment of central nervous system diseases.