马钱子苷对小胶质细胞活化的抑制作用

The inhibition of loganin on microglia activation

为探讨不同浓度的马钱子苷对小胶质细胞活化的抑制作用及其相关分子机制,分离培养了小鼠原代小胶质细胞,用不同浓度的马钱子苷(50、100、200μmol/L)预处理后,利用脂多糖(lipopolysacharide,LPS)诱导其活化。观察马钱子苷对LPS诱导的小胶质细胞活化、炎症因子释放以及NF-κB信号通路的影响。结果显示:与对照组相比,LPS组小胶质细胞胞体膨大,分泌大量炎症细胞因子,且NF-κB核转移程度增加(P<0.05),小胶质细胞呈明显的M1型活化状态;低、中、高浓度的马钱子苷干预后,小胶质细胞的细胞总面积和细胞核面积显著减少(P<0.05);中、高浓度的马钱子苷能使TNF-α、IL-6和IL-1β的mRNA表达水平降低(P<0.05);中浓度的马钱子苷下调TNF-α和IL-1β的蛋白质水平(P<0.05);此外低、中、高浓度的马钱子苷可以抑制小胶质细胞内iNOS表达和NF-κB信号通路激活。上述结果表明,马钱子苷可明显抑制小胶质细胞活化,减少炎症因子释放,其作用机制可能与抑制NF-κB信号通路有关。这为马钱子苷应用于神经炎症的预防和治疗奠定了理论基础。

This study aimed to investigate the inhibitory effects of different concentrations of loganin on microglia activation and its molecular mechanisms.In this study,primary mouse microglia were isolated and cultured.The cells were pretreated with different concentrations of loganin(50,100,and 200μmol/L),and then activated by lipopolysaccharide(LPS),to observe the effects of loganin on LPS-induced microglia activation,inflammatory factor release,and NF-κB signaling pathway.The results showed that,compared with the control group,the body of microglia in LPS group expanded,microglia secreted inflammatory cytokines in abundance,and the degree of nuclear transfer of NF-κB increased(P<0.05).Microglia showed obvious M1 activation state.After intervention with low,medium,and high concentrations of loganin glucoside,the total cell and nuclear areas of the microglia decreased significantly(P<0.05).In addition,low,medium,and high concentrations of loganin could inhibit iNOS expression and activation of NF-κB signaling pathway in microglia.These results suggest that loganin can significantly inhibit the activation of microglia and reduce the release of inflammatory factors,which may be related to the inhibition of NF-κB signaling pathway.This provides a theoretical basis for the application of loganin in the prevention and treatment of neuroinflammation.