辽宁省玉竹根腐病病原鉴定及生物学特性研究

Biological characterization and pathogen identification of Polygonatum odoratum root rot in Liaoning province

为鉴定辽宁省玉竹[PoLygonatum ordoratum(Mill.)Druce]根腐病病原菌的种类及其生物学特性,对玉竹根腐病病原菌进行了分离纯化培养,通过致病性测定,结合病菌形态学观察和延长因子(EF1-腐)序列分析,确定病原菌种类,并对病原菌的生物学特性进行了系统研究。结果表明,该病原菌为尖孢镰孢菌(Fusarium oxysporum),生长最适宜的培养基为马铃薯葡萄糖培养基,碳源为甘露醇,氮源为硝酸钾,温度为25~30℃,pH为5~7,光照条件为完全黑暗。

To identify the pathogen of Polygonatum odoratum root rot and clarify its biological characteristics in Liaoning province,which the pathogenic strains were isolated,purified and cultured.Based on pathogenicity tests,combining with morphological observation and extension factor(EF1-α)sequence analysis,the kinds of pathogenic strains were clarified and the biological characteristics of pathogen were systematically studied.The results indicated that the pathogen was identified as Fusarium oxysporum,which the optimum medium for pathogen growth was potato dextrose agar(PDA),the carbon source and nitrogen source was mannitol and potassium nitrate,respectively.The temperature was 25~30℃,pH was 5~7,and the light condition was completely dark.