摘要
设计了一个仪器分析实验,利用超高效合相色谱仪(Ultra Performance Convergence Chromatography,UPCC或UPC^(2))测定饮料中咖啡因的含量。该实验选取可乐、绿茶、乌龙茶三种饮料,样品用甲醇萃取,萃取液无需旋蒸,直接稀释定容后进样,测定其中咖啡因的含量。通过该实验学生不仅能巩固称量、定容、移液等基本操作,还能掌握UPC^(2)的基本原理、操作及应用色谱软件处理数据的技能。本实验将新型色谱仪UPC^(2)应用于基础实验教学,可以拓展学生的科学视野,有利于培养学生的创新精神和实践能力。
An experiment of instrumental analysis for determination of caffeine content in beverages by Ultra Performance Convergence Chromatography(UPC^(2))was designed.The caffeine in three beverages including coke,green tea and oolong tea was extracted and diluted by methanol.Then caffeine concentrations in beverages were measured by UPC^(2).Through this experiment,students could not only consolidate several laboratory skills such as weighing,constant volume and liquid transfer,but also master the basic theory,operation and data processing of UPC^(2).This experiment integrates the new chromatography into basic laboratory teaching,which can broaden students’scientific horizons.It is conductive to cultivating students’innovative spirit and practical ability.
作者
谢一凡
姚莉韵
严忠红
蔡玉兴
金玉杰
李宁
Yifan Xie;Liyun Yao;Zhonghong Yan;Yuxing Cai;Yujie Jin;Ning Li(Basic Medical Experimental Teaching Center,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China;Medicinal Chemistry and Bioinformatics Center,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China)
出处
《大学化学》
CAS
2021年第9期145-149,共5页
University Chemistry
基金
上海交通大学医学院课程建设基金项目(BJ13000180095)。
