摘要
推荐一个仪器分析创新实验——以市售邻苯二甲醛为底物采用光谱分析法检测还原型谷胱甘肽。内容涉及溶液配制、光谱分析及核磁共振氢谱和高分辨质谱测试。通过对本实验的实施,不仅可以让学生巩固分析化学、有机化学的相关基础知识,将其用于实际分析中;也可帮助他们了解科研前沿,培养科研能力和研究思路,激发学生的科研热情。
In this paper,an innovative experiment of instrumental analysis was recommended to detect reduced glutathione(GSH)by using commercial reagent o-phthalaldehyde.The experiment involves preparation of standard solutions,spectra analysis,and measurement of 1H nuclear magnetic resonance(NMR)spectroscopy and high resolution mass spectrometry(HR-MS).The implementation of this experiment can help students consolidate the basics knowledge of analytical chemistry and organic chemistry,understand the frontier scientific research,cultivate the scientific research ability and research ideas,and stimulate their enthusiasm for scientific research.
作者
张鹏
张倩
钟华
丁彩凤
Peng Zhang;Qian Zhang;Hua Zhong;Caifeng Ding(Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science,MOE,Shandong Key Laboratory of Biochemical Analysis,Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong,College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,Shandong Province,China)
出处
《大学化学》
CAS
2021年第9期150-156,共7页
University Chemistry
基金
国家自然科学基金(21904077,22074074,22004078)
山东省自然科学基金(ZR2019BB055)
厦门大学谱学分析与仪器教育部重点实验室(SCAI1702,SCAI1703)。
