手性气相色谱法测定野菊花中的龙脑对映体——推荐一个仪器分析实验

Determination of Borneol Isomer in Wild Chrysanthemum by Chiral Gas Chromatography:Recommending an Instrumental Analysis Experiment

推荐了一个仪器分析实验“手性气相色谱法测定野菊花中的龙脑对映体”。实验采用手性毛细管气相色谱柱,对野菊花中的(+)-龙脑和(−)-龙脑进行检测并定量。实验内容包括:野菊花挥发油的提取、色谱程序升温条件的优化、内标标准曲线法的应用等。本实验从分析化学的角度,重点介绍了手性色谱柱的种类及分离原理,建议作为拓展类的仪器分析实验开设。

An instrumental analysis experiment“Determination of borneol isomer in wild chrysanthemum by chiral capillary gas chromatography”is recommended.Determination and quantitative analysis of(+)-borneol and(−)-borneol in wild chrysanthemum were conducted using chiral capillary gas chromatographic column in this experiment.Experimental contents consisted of extraction of volatile oil of wild chrysanthemum,optimization of heating program,application of internal standard curve method,etc.From the perspective of analytical chemistry,this experiment focuses on the types and separation principle of chiral chromatographic columns,and it is suggested to be set up as an expanding instrumental analysis experiment.