猪流行性腹泻病毒与新发腹泻病毒混合感染分析

Co-infection Analysis of Porcine Epidemic Diarrhea Virus and Newly Emerged Diarrhea Viruses

为了分析猪德尔塔冠状病毒(PDCoV)、猪肠道α冠状病毒(PEAV)和猪流行性腹泻病毒(PEDV)在临床中的混合感染情况,采用反转录聚合酶链式反应(RT-PCR)方法将已经验证为PEDV阳性的样本进行PDCoV和PEAV检测。结果表明,PDCoV和PEDV混合感染比例为6.28%,191份PEDV阳性样本中,PDCoV阳性为12份,PEAV阳性为1份,PDCoV、PEAV和PEDV共感染的比例为0.52%。结果表明目前临床生产中PEDV仍然是引起仔猪腹泻的主要病毒性传染源,为临床生产中仔猪腹泻的防控工作提供理论依据和技术支撑。

In order to analysis co-infection of porcine epidemic diarrhea virus(PEDV),porcine deltacoronavirus(PDCoV)and porcine enteric alphacoronavirus(PEAV),RT-PCR was employed to detect the PDCoV and PEAV in PEDV-posivive samples.The results indicated that co-infection of PDCoV and PEDV was 6.28%,PDCoV positive sample was 12,PEAV positive sample was 1,and co-infection of PEDV,PDCoV and PEAV was 0.52%.The present study showed that PEDV was currently the major viral pathogen causing diarrhea in sucking pigs.The information obtained in the present study will provide theoretical basis and technical support for the control and prevention of clinical diarrhea of sucking pig.