摘要
将雨生红球藻中的β-胡萝卜素酮化酶基因(bkt)和β-胡萝卜素羟化酶基因(crtR-B)经密码子优化后,通过自然转化法分别转入集胞藻PCC 6803基因组中。高效液相色谱分析显示:转入bkt基因的细胞产生角黄质的同时,海胆酮含量减少;转入crtR-B基因的细胞产生金盏花黄质的同时,玉米黄素含量减少。实验结果表明,外源的β-胡萝卜素酮化酶基因将海胆酮转化为角黄质,外源的β-胡萝卜素羟化酶基因将玉米黄素转化为金盏花黄质。该文利用代谢工程策略,在集胞藻PCC 6803中构建类胡萝卜素生物合成途径,为通过代谢工程在集胞藻PCC 6803中生产虾青素奠定了基础。
Theβ-carotene ketolase gene(bkt)andβ-carotene hydroxylase gene(crtR-B)from Haematococcus pluvialis were codon-optimized and transferred to Synechocystis sp.PCC 6803 genome by natural transformation method.High performance liquid chromatography analysis showed that cells transfected with bkt gene produced canthaxanthin,while echinone decreased;the cells with crtR-B gene produced adonixanthin,while zeaxanthin was reduced.The results showed that the exogenousβ-carotene ketolase converted echinone to canthaxanthin and the exogenousβ-carotene hydroxylase converted zeaxanthin into adonixanthin.In this paper,the pathway of astaxanthin biosynthesis in Synechocystis sp.PCC 6803 was constructed by metabolic engineering strategy,which laid a foundation for astaxanthin production in Synechocystis sp.PCC 6803 with metabolic engineering.
作者
刘亚铭
王康
崔玉琳
陈高
秦松
LIU Yaming;WANG Kang;CUI Yulin;CHEN Gao;QIN Song(Key Laboratory of Coastal Biology and Biological Resource Utilization,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;University of Chinese Academy of Sciences,Beijing 101418,China;Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China;Institute of Crop Germplasm Resources,Shandong Academy of Agricultural Sciences,Jinan 250100,China;Shandong Provincial Key Laboratory of Academy Crop Genetic Improvement,Ecology and Physiology,Jinan 250100,China)
出处
《集成技术》
2021年第5期96-103,共8页
Journal of Integration Technology
基金
国家自然科学基金项目(41876188)
山东省自然科学基金项目(ZR2018ZB0210)。
