拮抗miR-21对人工合成高效孕激素作用下子宫内膜癌细胞增殖和凋亡的影响

Effect of antagonistic miR-21 combined with synthetic high-efficiency progesterone on the proliferation and apoptosis of endometrial cancer cells

目的探讨拮抗miR-21联合人工合成高效孕激素对子宫内膜癌Ishikawa细胞增殖、凋亡的影响。方法采用流式细胞法、细胞计数法(CCK-8)、Transwell实验及划痕实验检测各组Ishikawa细胞的凋亡、增殖、侵袭及迁移能力;实验分为两组。空白细胞组:未经任何处理的子宫内膜Ishikawa细胞;高效孕激素组:经人工合成高效孕激素处理的子宫内膜Ishikawa细胞;高效孕激素+miR-21组:经人工合成高效孕激素和miR-21拮抗剂共同处理的子宫内膜Ishikawa细胞。结果干预前与干预24 h后高效孕激素组细胞增殖率、高效孕激素+miR-21组细胞增殖率比较差异有统计学意义(P<0.05);高效孕激素+miR-21组细胞增殖率与其他2组比较差异有统计学意义(P<0.05);干预前与干预24 h后高效孕激素组细胞迁移率、高效孕激素+miR-21组细胞迁移率比较差异有统计学意义(P<0.05);高效孕激素+miR-21组细胞迁移率与其他2组比较差异有统计学意义(P<0.05);干预前与干预24 h后高效孕激素组细胞侵袭力、高效孕激素+miR-21组细胞侵袭力比较差异有统计学意义(P<0.05);高效孕激素+miR-21组细胞侵袭力与其他2组比较差异有统计学意义(P<0.05);干预前与干预24 h后高效孕激素组细胞凋亡率、高效孕激素+miR-21组细胞凋亡率比较差异有统计学意义(P<0.05);高效孕激素+miR-21组细胞凋亡率与其他2组比较差异有统计学意义(P<0.05)。结论拮抗miR-21可促进人工合成高效孕激素作用下Ishikawa细胞的凋亡及抑制其增殖。

Objective To investigate the effect of antagonistic miR-21 combined with synthetic high-efficiency progesterone on the proliferation and apoptosis of endometrial cancer cells.Methods Flow cytometry,cell counting method(CCK-8),Transwell test and scratch test were used to detect the apoptosis,proliferation,invasion and migration ability of endometrial ishikawa cells.The endometrial ishikawa cells were divided into three groups,including control group(without any treatment),high-efficiency progesterone group(the cells treated with synthetic high-efficiency progesterone)and high-efficiency progesteronet combined with miR-21 group(the cells treated with synthetic high-efficiency progesterone and miR-21 antagonist).Results The cell proliferation rate of high-efficiency progesterone group was significantly different from that before intervention(P<0.05).The cell proliferation rate of high-efficiency progesterone+miR-21 group was significantly different from that before intervention(P<0.05).Compared with the other two groups,the cell proliferation rate of high efficiency progestin+miR-21 group was the lowest,and the difference was statistically significant(P<0.05).The cell migration rate of high-efficiency progestin group was significantly different from that before intervention(P<0.05).The cell migration rate of high-potency progesterone+miR-21 group was significantly different from that before intervention(P<0.05).Compared with the other two groups,the cell migration rate of high-potency progestin+miR-21 group was the lowest,and the difference was statistically significant(P<0.05).The cell invasiveness of high-efficiency progesterone group was significantly different from that before intervention(P<0.05).The invasiveness of high-potency progesterone+miR-21 group was significantly different from that before intervention(P<0.05).Compared with the other two groups,the high-potency progesterone+miR-21 group had the lowest cell invasion,and the difference was statistically significant(P<0.05).The apoptosis rate of high-efficiency progesterone group was significantly different from that before intervention(P<0.05).The apoptosis rate of high-efficiency progesterone+miR-21 group was significantly different from that before intervention(P<0.05).Compared with the other two groups,the apoptosis rate of high efficiency progestin+miR-21 group was the highest and the difference was statistically significant(P<0.05).Conclusion Antagonistic miR-21 combined with synthetic high-efficiency progesterone can promote the apoptosis and inhibit the proliferation of endometrial cancer ishikawa cells.