摘要
Dengue virus is an arthropod-borne pathogen that is transmitted to humans primarily by Aedes spp.mosquitos,causing the acute infectious disease,dengue fever(DF).Until 2019,no dengue outbreak had been reported in Hainan Province for over 20 years.However,in early September of 2019,an increasing number of infected cases appeared and the DF outbreak lasted for over one month in Haikou City,Hainan Province.In our study,we collected 97 plasma samples from DF patients at three hospitals,as well as 1585 mosquito larvae samples from puddles in different areas of Haikou.There were 49(50.5%)plasma samples found to be strongly positive and 9(9.3%)plasma samples were weakly positive against the NS1 antigen.We discovered DENV both in the patient's plasma samples and mosquito larvae samples,and isolated the virus from C6/36 cells inoculated with the acute phase serum of patients.Phylogenetic analysis revealed that the new strains were the most closely related to the epidemic strain in the southern regions of China,belonging to lineageⅣ,genotypeⅠ,DENV-1.Compared to the seven closest strains from neighboring countries and provinces,a total of 18 amino acid mutations occurred in the coding sequences(CDS)of the new isolated strain,DENV1 HMU-HKU-2.Our data shows that dengue virus is re-emerged in Hainan,and pose new threats for public health.Thus regular molecular epidemiological surveillance is necessary for control and prevention of DENV transmission.
基金
This work was supported by the Hainan Provincial Natural Science Foundation of China(Grant No.2019RC218)
the National Natural Science Foundation of China(grant No.81860367,31460017)
the Scientific Research Foundation of the Higher Education Institutions of Hainan Province,China(Grant No.Hnky2020-33)
the Hainan Medical University novel coronavirus pneumonia project(Grant No.XGZX2020005)
the Hainan Provincial Natural Science Foundation of China(Grant No.819QN360)
State Key Laboratory of Virology 2018 Open Fund Project(2018IOV002)
the National S&T Major Project"China Mega-Project for Infectious Disease"(Grant No.2018ZX10711001)
the CAMS Innovation Fund for Medical Sciences(Grant No.2017-I2M-3-017).
