摘要
Muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation.A growing body of evidence suggests that microRNAs play critical roles in myocyte proliferation and differentiation transcriptionally.However,the molecular mechanisms underlying the orchestration are not fully understood.Here,we showed that miR-130b is able to repress myoblast proliferation and promote myogenic differentiation via targeting Sp1 transcription factor.Importantly,overexpression of miR-130b is capable of improving the recovery of damaged muscle in a freeze injury model.Moreover,miR-130b expression is declined in the muscle of muscular dystrophy patients.Thus,these results indicated that miR-130b may play a role in skeletal muscle regeneration and myopathy progression.Together,our findings suggest that the miR-130b/Sp1 axis may serve as a potential therapeutic target for the treatment of patients with muscle damage or severe myopathies.
基金
This work was supported by grants from the National Natural Science Foundation of China(81570768 to Y.-C.W.,31900841 to Yan Li,91957205 and 31525012 to H.Y.,and 81471016 to J.J.)
the Ministry of Science and Technology of China(2016YFA0500102 and 2016YFC1304905)
CAS Key Laboratory of Nutrition,Metabolism and Food Safety(KLNMFS2019-01),and Chinese Academy of Sciences Interdisciplinary Innovation Team.