FOXO转录因子亚家族在瘢痕疙瘩中的表达及意义初探

Expression and Significance of FOXO Transcription Factor Subfamily in Keloid

目的检测人类叉头框O(FOXO)转录因子亚家族(FOXO1、FOXO3a、FOXO4、FOXO6)在人瘢痕疙瘩组织中的表达,为下一步探讨FOXO转录因子亚家族对瘢痕疙瘩生物学活性的影响提供实验依据。方法通过实时荧光定量PCR、蛋白免疫印迹法以及免疫组织化学染色法检测FOXO转录因子亚家族中FOXO1、FOXO3a、FOXO4、FOXO6在人瘢痕疙瘩组织中的表达情况。结果实时荧光定量PCR、蛋白免疫印迹法以及免疫组织化学染色法均证实与正常皮肤组织相比FOXO1、FOXO3a、FOXO4在瘢痕疙瘩组织中的表达均显著降低,差异具有统计学意义(P<0.01),FOXO6的表达差异无统计学意义(P>0.05)。结论瘢痕疙瘩中显著低表达的FOXO1、FOXO3a、FOXO4可能对瘢痕疙瘩的生物学活性有影响。

Objective To study the expression of human forkhead box O(FOXO)transcription factor subfamily members(FOXO1,FOXO3a,FOXO4,FOXO6)in human keloid tissue,and to provide experimental basis for further exploring the effect of FOXO transcription factor subfamily on the biological activity of keloid.Methods The expression of FOXO1,FOXO3a,FOXO4 and FOXO6 in the subfamily of FOXO transcription factor in human keloid tissues was detected by quantitative real-time PCR,Western blot and immunohistochemistry.Results Quantitative real-time PCR,Western blot and immunohistochemical staining all confirmed that the expression of FOXO1,FOXO3a and FOXO4 in keloid tissue was significantly decreased compared with normal skin tissue,and the difference was statistically significant(P<0.01).There was no significant(P>0.05)difference in the expression of FOXO6.Conclusion The significantly low expression of FOXO1,FOXO3a and FOXO4 in keloid may affect the biological activity of keloid.