利用InDel标记分析中国香菇菌株的遗传多样性与群体结构

Genetic diversity and population structure of Lentinula edodes analyzed by InDel markers

通过对5个香菇菌株重测序,以香菇L808-1菌株的全基因组序列为参考基因组,分析了这些菌株中插入/缺失(In Del)标记位点在香菇基因组10条染色体上的分布,并筛选了插入/缺失碱基数≥15的位点,合成了449对In Del标记引物。经过PCR和电泳检测,其中237对引物条带清晰。最终筛选出107个PIC≥0.3的标记作为核心In Del标记对来自国内的44份香菇菌株进行遗传多样性分析。聚类分析显示栽培菌株和野生菌株各自聚为一支,所选香菇菌株间存在明显的群体分层。群体结构分析显示香菇种质资源可分为4个亚群,主成分分析显示香菇菌株之间的位置及距离与聚类分析和群体结构分析结果相符。香菇In Del分子标记的开发与应用,为香菇核心种质的构建和种质资源育种引用提供了基础。

Based on resequencing five strains of Lentinula edodes and mapping the whole genome sequence of monokaryon strain L808-1,the insertion/deletion(InDel)markers on 10 chromosomes of L.edodes genome were analyzed.Loci with more than 15 InDel bases were selected,and 449 pairs of InDel primers were synthesized.PCR and electrophoresis results revealed that 237 pairs among them showed clear bands,and 107 pairs(PIC≥3)were screened out as core InDel markers,which were later applied to analyze the genetic diversity of 44 L.edodes strains.Cluster analysis grouped the cultivars and wild strains into individual branches respectively,indicating the obvious population stratification among them.Population structure analysis categorized the germplasm resources of L.edodes into four subgroups.Principal component analysis showed that the position and distance between strains were consistent with the results of cluster analysis and population structure analysis.The InDel markers developed and applied in this paper provide a basis for the construction of L.edodes core germplasm and effective breeding of germplasms.