摘要
通过对5个香菇菌株重测序,以香菇L808-1菌株的全基因组序列为参考基因组,分析了这些菌株中插入/缺失(In Del)标记位点在香菇基因组10条染色体上的分布,并筛选了插入/缺失碱基数≥15的位点,合成了449对In Del标记引物。经过PCR和电泳检测,其中237对引物条带清晰。最终筛选出107个PIC≥0.3的标记作为核心In Del标记对来自国内的44份香菇菌株进行遗传多样性分析。聚类分析显示栽培菌株和野生菌株各自聚为一支,所选香菇菌株间存在明显的群体分层。群体结构分析显示香菇种质资源可分为4个亚群,主成分分析显示香菇菌株之间的位置及距离与聚类分析和群体结构分析结果相符。香菇In Del分子标记的开发与应用,为香菇核心种质的构建和种质资源育种引用提供了基础。
Based on resequencing five strains of Lentinula edodes and mapping the whole genome sequence of monokaryon strain L808-1,the insertion/deletion(InDel)markers on 10 chromosomes of L.edodes genome were analyzed.Loci with more than 15 InDel bases were selected,and 449 pairs of InDel primers were synthesized.PCR and electrophoresis results revealed that 237 pairs among them showed clear bands,and 107 pairs(PIC≥3)were screened out as core InDel markers,which were later applied to analyze the genetic diversity of 44 L.edodes strains.Cluster analysis grouped the cultivars and wild strains into individual branches respectively,indicating the obvious population stratification among them.Population structure analysis categorized the germplasm resources of L.edodes into four subgroups.Principal component analysis showed that the position and distance between strains were consistent with the results of cluster analysis and population structure analysis.The InDel markers developed and applied in this paper provide a basis for the construction of L.edodes core germplasm and effective breeding of germplasms.
作者
沈秀芬
章炉军
张美彦
张丹
于海龙
李玉
SHEN Xiu-Fen;ZHANG Lu-Jun;ZHANG Mei-Yan;ZHANG Dan;YU Hai-Long;LI Yu(Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun,Jilin 130118,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,Shanghai 201403,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2021年第9期2266-2281,共16页
Mycosystema
基金
上海市科技兴农项目(2019-02-08-00-08-F01114)
国家现代农业产业技术体系(CARS-20)
上海市农业科学院卓越团队[2017(A-02)]。
关键词
香菇
重测序
INDEL标记
遗传多样性
群体结构
Lentinula edodes
re-sequencing
InDel markers
genetic diversity
population structure