灵芝子实体多糖对小鼠急性酒精肝损伤预防的代谢组分析

Preventive effects of the Ganoderma lingzhi fruit-body polysaccharides on the acute alcoholic injury of mice liver based on metabonomics analysis

灵芝多糖是灵芝的主要药理活性成分之一。本研究通过检测血清指标分析灵芝子实体多糖(Ganoderma lingzhi fruitbody polysaccharides,GLFPS)对小鼠急性酒精性肝损伤的预防作用并结合代谢组学探究作用机制。结果显示,GLFPS显著抑制因酒精作用而升高的小鼠血清中ALT、AST、TG、TC和ADH水平。通过代谢组分析,在模型组与对照组中得到85个差异代谢物,其中三磷酸腺苷(adenosine triphosphate)、L-天门冬氨酸(L-aspartic acid)以及赖氨酸(L-lysine)等在相互作用网络中起重要作用,说明酒精能引起小鼠肝脏腺苷和氨基酸代谢的改变。GLFPS组与模型组有58个差异代谢物,主要包括脂质和有机氧化物,说明GLFPS可以通过调节小鼠肝脏中脂质与有机化合物代谢来预防急性酒精肝损伤。对差异代谢物进行KEGG富集分析发现主要涉及胆碱代谢、甘油磷脂代谢和ABC转运蛋白。GLFPS能够有效缓解这3个代谢通路中因酒精作用发生明显改变的代谢。综上可见,灵芝子实体多糖能够通过调节小鼠胆碱代谢、甘油磷脂代谢以及部分ABC转运蛋白有效预防酒精性肝损伤。

Polysaccharide is one of the main pharmacological active components of Ganoderma lingzhi.The preventive effect of polysaccharides from G.lingzhi fruit-body(GLFPS)on acute alcoholic liver injury in mice was analyzed by detecting serum indexes,and the mechanism of metabonomics was explored.The results showed that GLFPS significantly inhibited the levels of ALT,AST,TG,TC and ADH in serum of mice induced by alcohol.With the help of metabonomics analysis,85 different metabolites were obtained between the model group and the control group.Among them,adenosine triphosphate,L-aspartic acid,and L-lysine play important roles in the interaction network,indicating that alcohol can cause changes in the metabolism of adenosine and amino acids in the liver of mice.There were 58 different metabolites between the GLFPS treated group and the model group,mainly including lipids and organic oxygen.KEGG enrichment analysis of different metabolites showed that they were involved in choline metabolism,glycerophospholipid metabolism,and ABC transporters.GLFPS can effectively relieve hurt of these three metabolic pathways significantly changed by alcohol.In conclusion,GLFPS effectively prevent alcoholic liver injury by regulating the metabolism of choline,glycerophospholipids,and some ABC transporters in mice.