垂花百合花色变异机制的转录组学分析

Transcriptomic Analysis of the Mechanism of Flower Color Variation in Lilium cernum

为探求两种不同花色类型的垂花百合花色变异机制,本研究以相同生境下不同花色的垂花百合(Lilium cernuum)花瓣为材料,利用高通量测序技术建立了花发育过程和两种花色类型的转录组数据库,并针对花色苷合成代谢途径的结构基因和转录因子进行差异筛选。结果表明,测序共获得57521条Unigene,对比不同阶段花器官中花色苷合成的结构基因表达差异情况,筛选出DFR、CHS、ANR、ANS和FOMT共5条结构基因,以及MYB14、MYB98、MYBC1、PHL7、PHL8、细胞色素P45094A1、bHLH30、bHLH51、MYB13、bHLH52、bHLH35共11条转录因子,这些结构基因和转录因子被推测可能与花色苷合成调节有关。本研究认为垂花百合粉白型花色可能是在转色期转色阶段未完全完成时就进行开花过程导致。

In order to explo re the variation mechanism of two different colors of Lilium cernuum,the floral organs of Lilium cernuum with different colors and same habitat were used as materials.A high-throughput sequencing technique applied used to establish the process of flower development and the transcriptome database of the two types of flowers.The structural genes and transcription factors of anthocyanin biosynthesis pathway were screened differentially.The results revealed that a total of 57521 Unigenes were obtained by sequencing.Compared to the expression differences of structural genes of anthocyanin synthesis in floral organs at different stages,5 structural genes were screened out,including DFR,CHS,ANR,ANS and FOMT,and 11 transcription factors,such as MYB14,MYB98,MYBC1,PHL7,PHL8,cytochrome P45094 A1,bHLH30,bHLH51,MYB13,bHLH52 and b HLH35.These structural genes and transcription factors are speculated to be closely related to the regulation of anthocyanin synthesis.At the same time,it is believed that pink Lilium cernuum may be caused by the flowering process,during which the color conversion stage is not completely completed.