葡萄GRF基因家族的生物信息学分析

Bioinformatics Analysis of GRF Gene Family in Grape

GRF(Growth-regulating factor)基因家族是一种广泛分布于种子植物的转录因子,在调节植物的生长、发育中发挥重要的作用。本研究从葡萄基因组鉴定出8个VvGRF基因,命名为VvGRF1~VvGRF8。采用生物信息学的方法,系统地对葡萄Vv GRF家族的蛋白理化性质、进化关系、保守基序及在不同发育时期的差异性表达进行预测和分析。结果表明,VvGRF基因内含子与外显子数量差异较小,在所有预测蛋白的N端含有两个典型的保守结构域QLQ与WRC;系统进化分析将GRF家族成员分为6组,其中葡萄与甜瓜的亲缘关系较拟南芥、番茄两种双子叶植物近;顺式作用元件表明,调控分生组织、胚、种子发育及生长素、赤霉素等信号传导和逆境胁迫响应元件,在VvGRFs启动子序列中大量存在;基因特异性表达分析显示,多数GRF基因在葡萄发育的花后2周、10周时期的表达丰富度高,在接近成熟时期的表达丰富度较低,表明GRF在葡萄生长、转色早期起着重要的作用,参与组织、器官的生长调节。本研究为进一步探究葡萄GRF基因家族的结构及生长发育时期的功能调节提供了参考依据。

The GRF(Growth-regulating factor)gene family is a transcription factor widely distributed in seed plants and plays an important role in regulating plant growth and development.In this study,eight VvGRF genes were identified from the grape genome,named VvGRF1~VvGRF8.Using bioinformatics methods,systematically predict and analyze the physical and chemical properties,evolutionary relationships,conservative motifs,and differential expressions of the grape Vv GRF family proteins at different developmental stages.The results showed that the number of introns and exons of VvGRF gene is small,and there are two typical conserved domains QLQ and WRC at the N-terminus of all predicted proteins;Phylogenetic analysis divided the members of the GRF family into 6 groups,among which the genetic relationship between grapes and melons is closer than that of Arabidopsis and tomato;The cis-acting elements indicate that the signal transduction and stress response elements that regulate meristem,embryo,seed development,auxin,gibberellin,etc.,are abundantly present in the VvGRFs promoter sequence;Gene-specific expression analysis showed that most GRF genes have high expression richness in the 2 and 10 weeks after grape development,and low expression richness in the near mature period,indicating that GRF plays a role in the early stage of grape growth and color transformation.It plays an important role in the growth regulation of tissues and organs.This study provides a reference for further exploring the structure of grape GRF gene family and the functional regulation during growth and development.