不同浓度K^(+)处理下黑果枸杞K^(+)通道相关基因的表达水平分析

Expression Level Analysis of K^(+)Channel Related Genes in Lycium ruthenicum under Different K^(+)Concentrations

本研究利用不同浓度的K^(+)(KCl)溶液对‘青黑杞1号’的组培幼苗进行处理,检测和分析与K^(+)通道相关的基因在黑果枸杞中的表达模式。采用RT-PCR等方法,分析在不同浓度K^(+)条件下,枸杞中K^(+)通道相关基因的表达量的变化情况。结果显示:(1)100、250和300 mmol/L K^(+)处理后,黑果枸杞中K^(+)通道相关基因的表达水平较低;(2)当外界K^(+)浓度大于300 mmol/L时,部分基因的表达量上升,随着外界K^(+)浓度的继续增加,黑果枸杞的KCO1B基因表达水平先下降后上升,而Cluster-17443.69422的表达水平先上升后下降,TPK1基因、KT1基因、Cluster-17443.10870基因和Cluster-17443.15033基因的表达量则呈下降、上升再下降的趋势。本研究结果为研究黑果枸杞钾盐胁迫调节机制提供了理论基础。

In this study,different concentrations of KCl solutions were used to detect and analyze the expression patterns of potassium channel related genes in’Qingheiqi’No.1.According to the results of RT-PCR,the expression of potassium channel related genes in Lycium was analyzed.The results showed that:(1)After K^(+)treatment of 100,250 and 300 mmol/L,the expression level of potassium channel related genes in lycium barbarum is low.(2)When the concentration of external potassium ion is greater than 300 mmol/L,the expression of some genes increases.With the continuous increase of external potassium concentration,the expression level of KCO1 B gene decreases first and then increases.However,the expression levels of Cluster-17443.69422 first increases and then decreases,while the expressions of TPK1,KT1,Cluster-17443.10870 and Cluster-17443.15033 decreases,increases and then decreases.The results of this study provide a theoretical basis for the study of potassium stress regulation mechanism of lycium barbarum.