鸡蛋花组织培养的快速繁殖技术

A Study on Tissue Culture and Rapid Propagation of Plumeria rubra L.

为了获得鸡蛋花组织培养快速繁殖技术体系,本研究通过鸡蛋花顶芽诱导繁殖的组织培养方法,以鸡蛋花种子播种的无菌苗顶芽作为组织培养诱导的外植体,在诱导培养基中诱导产生丛生芽,再在芽诱导培养基中进行继代增殖,培养出大量的丛生芽后,再经过壮苗生根,炼苗阶段,直接移栽到培养杯,获得了鸡蛋花组培苗。通过3次重复试验,获得一套鸡蛋花组织培养快速繁殖体系,诱导率达到100%,丛生芽个数达到10个左右,生根率达到100%,炼苗的成活率达到97%,此成果更接近工厂化育苗和产业化的要求,为鸡蛋花组培苗工厂化育苗和产业化生产提供技术的支持。

In order to obtain the rapid propagation technology system of Plumeria rubra L.Thunb,a rapid propagation method for Plumeria rubra L.terminal bud induction via tissue culture was studied.Terminal buds from seedlings of sterile Plumeria rubra L.seeds were used as explants,and adventitious buds were directly induced in bud induction medium.After alternate propagation culture in bud induction medium,a large number of cluster buds were obtained.After rooting and refining,plantlets were directly transplanted to culture cup,and the tissue culture plantlets of Plumeria rubra L.were obtained.Through three repeated experiments,a set of rapid propagation system of frangipani tissue culture was obtained.The induction rate reached 100%,the number of cluster buds per plant reached about 10,the rooting rate reached 100%,and the survival rate of refined seedlings reached 97%.To provide technical support for industrialization production and industrialization development on tissue culture seedlings of Plumeria rubra L..