响应面优化微波辅助提取魔芋多糖的工艺

Optimization of Konjac Polysaccharide Extraction from Amorphophallus konjac by Response Surface Methodology

以魔芋为原料,以水为提取溶剂,以魔芋多糖提取率为评价指标,旨在考察微波功率、提取时间、料液比对魔芋多糖提取率的影响。在单因素试验的基础上,采用Box-Behnken响应面法优化魔芋多糖的提取工艺条件。结果表明,最佳提取条件为微波功率420 W、提取时间35 min、料液比1︰250 (g/mL)。在此条件下魔芋多糖的提取率为17.35%。试验结果说明采用响应面法优化微波辅助提取魔芋多糖的提取工艺可行。

Using konjac as raw material,water as extraction solvent and the extraction rate of konjac polysaccharide as evaluation index,the effects of microwave power,extraction time and solid-liquid ratio on the extraction rate of konjac polysaccharide were investigated.On the basis of single factor experiment,Box-Behnken response surface methodology was used to optimize the extraction condition of konjac polysaccharide.The results showed that the optimal extraction condition was as follows:microwave power 420 W,extraction time 35 min and solid-liquid ratio 1︰250(g/mL).Under this condition,the extraction rate of konjac polysaccharide was 17.35%.The results indicated that it was feasible to optimize the microwave-assisted extraction process of konjac polysaccharide by response surface methodology.