液质联用法测定掺有复原乳的巴氏杀菌乳中的糠氨酸

Determination of Furosine in Pasteurized Milk Mixed with Reconstituted Milk by LC-MS/MS

试验以液相色谱串联质谱法测定巴氏杀菌乳中的糠氨酸含量,分析在其生产过程中是否掺入复原乳。将不同品牌的奶粉溶解成复原乳后以不同比例掺入生鲜乳中,经巴氏杀菌后测定糠氨酸含量。结果表明,在稳定、可靠的杀菌加热条件下,随着巴氏杀菌乳中掺入复原乳比例升高,测得的糠氨酸含量呈线性增长趋势;不同品牌复原乳掺入生鲜乳中,掺入比例5%时,糠氨酸含量与未掺入复原乳样品表现出极显著差异(p<0.01)。液质联用法测定糠氨酸可以提供一种灵敏的、分析巴氏杀菌乳中是否掺入复原乳的方法。

The liquid chromatography tandem mass spectrometry was used to determine the content of furosine in pasteurized milk to analyze whether reconstituted milk was incorporated in liquid milk production.Different brands of milk powder were dissolved into reconstituted milk and then blended into raw milk in different proportions.The content of furosine was determined after pasteurization and heating.The results showed that the measured pasteurized milk increased with the proportion of blended reconstituted milk,and its furosine content showed a linear increase;When different brands of reconstituted milk were blended into raw milk in the case of 5% blending,the content of furosine and the sample without reconstituted milk showed a very significant difference.The determination of furosine by liquid mass spectrometry could provide a more sensitive method to differentiate whether pasteurized milk was mixed with reconstituted milk.