负载仿生矿化前驱体的大孔径介孔硅介导的胶原纤维内仿生矿化的研究

Intrafibrillar biomineralization mediated by large pore mesoporous silica loaded with biomimetic mineralization precursors

目的:应用大孔径介孔硅负载并转运仿生矿化前驱体,对Ⅰ型胶原进行纤维内仿生矿化,探索从液体介导的仿生矿化理念向以纳米颗粒介导的仿生矿化的转化。方法:制备氨基功能化的大孔径介孔硅(amine functionalized large pore mesoporous silica nanoparticles, AF-LPMSN),并负载无定形磷酸钙仿生矿化前驱体Pasp-ACP,以获得Pasp-ACP@AF-LPMSN转运体系。通过透射电镜、傅里叶红外光谱和电感耦合等离子体发射光谱仪检测Pasp-ACP@AF-LPMSN形貌、物理化学性能及仿生矿化前驱体释放动力学;通过2D胶原纤维网仿生矿化实验,检测Pasp-ACP@AF-LPMSN对Ⅰ型胶原进行纤维内仿生矿化的性能;通过Pasp-ACP@AF-LPMSN与人牙髓干细胞共培养检测生物相容性。结果:Pasp-ACP@AF-LPMSN能负载及释放无定形磷酸钙仿生矿化前驱体,并促进Ⅰ型胶原发生纤维内均质矿化,具有良好的生物相容性。结论:Pasp-ACP@AF-LPMSN转运体系可以促进Ⅰ型胶原纤维内仿生矿化,具有在口腔硬组织再矿化领域应用的潜能。

Objective:To explore the translation from the concept of liquid mediated biomineralization to nano-particle mediated biomineralization, by employing large pore mesoporous silica to deliver biomimetic mineralization precursors and mediate intrafibrillar biomineralization of type Ⅰ collagen.Methods:Amine functionalized large pore mesoporous silica nanoparticles(AF-LPMSN)were prepared and loaded with poly-aspartic acid-amorphous calcium phosphate(Pasp-ACP)to obtain Pasp-ACP@AF-LPMSN transport system.Transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FTIR),and inductively coupled plasma emission spectrometer(ICP-AES)were used to detect the physical and chemical properties of Pasp-ACP@AF-LPMSN and the release kinetics of biomimetic mineralization precursors.The intrafibrillar biomineralization performance of Pasp-ACP@AF-LPMSN was detected by biomineralization of type Ⅰ collagen.Biocompatibility was detected by co-culture of Pasp-ACP@AF-LPMSN with human dental pulp stem cells.Results:Biomimetic mineralization precursors can be delivered by the Pasp-ACP@AF-LPMSN transport system, which can not only promote the intrafibrillar biomineralization of type Ⅰ collagen but also has good biocompatibility.Conclusion:The Pasp-ACP@AF-LPMSN delivery system can promote the intrafibrillar biomineralization of type Ⅰ collagen and has the potential of application in the field of oral hard tissue remineralization.