摘要
在针对长非编码RNA(lncRNA)进行CRISPR/Cas9基因编辑时,从头设计有效singleguide RNA(sgRNA)并非易事,因此收集了已经发表文章中经实验验证过的sgRNA信息,并基于MTV模式的Django框架,开发出CRISPRlnc网站数据库。该数据库的开发将为科研工作者针对lncRNA进行CRISPR/Cas9基因编辑提供有效的辅助,并促进lncRNA的功能研究和CRISPR/Cas9技术的发展。
It is not easy to design effective singleguideRNAs(sgRNAs)from scratch in CRISPR/Cas9 gene editing for long non coding RNAs(lncRNAs),so the sgRNAs information verified by experiments in published articles is collected,and the CRISPRlnc website database based on the Django framework of MTV pattern is developed.The CRISPRlnc will not only provide a effective assistance for researchers using CRISPR/Cas9 to edit lncRNAs,but also promote the functional study of lncRNAs and the development of CRISPR/Cas9 technology.
作者
张国强
刘长宁
Zhang Guoqiang;Liu Changning(Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences,Xishuangbanna,Yunnan 666303,China;University of Chinese Academy of Sciences)
出处
《计算机时代》
2021年第10期32-34,40,共4页
Computer Era
基金
国家自然科学基金“基于多组学数据整合的肿瘤相关长非编码RNA优化筛选和调控机制研究”(31471220)。
