基于超连续谱光源的相干反斯托克斯拉曼散射成像理论与实验可行性研究

Theoretical and Experimental Studies on Coherent Anti-Stokes Raman Scattering Microscopy Based on Supercontinuum Fiber Laser

将超连续谱光纤激光器引入到相干反斯托克斯拉曼散射成像系统,以解决当前相干反斯托克斯拉曼散射成像系统造价昂贵、体积庞大的问题。首先分析了基于超连续谱光源的相干反斯托克斯拉曼散射成像理论及其影响因素;接着搭建了一套基于超连续谱光源的相干反斯托克斯拉曼散射成像系统,并进行了成像性能测试。在物镜、功率、浓度等不同影响因素的情况下,系统测试的实验结果与数值仿真结果具有高度一致性,验证了基于超连续谱光源的相干反斯托克斯拉曼散射成像理论与实验的可行性。

A coherent anti-Stokes Raman scattering microscope is built by using a supercontinuum fiber laser,towards a solution of the disadvantages in current imaging system,such as the cost and bulk-size.Firstly,the theory of supercontinuum fiber laser-based coherent anti-Stokes Raman scattering imaging is deduced and its influencing factors are analyzed. An experimental setup for the supercontinuum fiber laserbased coherent anti-Stokes Raman scattering microscope is then built up,while its experimental feasibility is demonstrated. Different influencing factors,including objective lens,laser power and concentration,are selected to demonstrate that the test results are highly consistent with theoretical ones. The results prove the theoretical and experimental feasibility of coherent anti-Stokes Raman scattering microscopy based on supercontinuum fiber laser.