内脂素对肺癌细胞凋亡的影响及对PI3K/AKT信号通路的作用研究

Effect of visfatin on lung cancer cell apoptosis and its effect on PI3K/AKT signaling pathway.

目的探讨与研究内脂素对肺癌细胞凋亡的影响及对磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)信号通路的作用。方法对数生长期的A549细胞,平分为3组:对照组、内脂素A组与内脂素B组。对照组加入无血清RPMI-1640培养基,内脂素A组加入10μg/mL内脂素作用于细胞60 min,内脂素B组加入20μg/mL内脂素作用于细胞60 min。采用CCK-8法检测细胞增殖,双染法检测细胞凋亡,Transwell实验检测细胞侵袭,蛋白质印迹法检测PI3K/AKT蛋白表达。结果处理后24、36 h,内脂素B组与内脂素A组的细胞增殖指数、过膜细胞数高于对照组,内脂素B组也显著高于内脂素A组,差异均有统计学意义(P<0.05)。内脂素B组与内脂素A组的细胞凋亡率显著低于对照组,内脂素B组也显著低于内脂素A组,差异均有统计学意义(P<0.05)。内脂素B组与内脂素A组的PI3K、AKT蛋白相对表达水平显著高于对照组,内脂素B组也显著高于内脂素A组,差异均有统计学意义(P<0.05)。结论内脂素在肺癌细胞的应用能抑制细胞凋亡,促进细胞增殖与侵袭,其作用机制可能与PI3K/AKT信号通路的激活有关。

Objective To investigate and study the effect of endolipin on lung cancer cell apoptosis and the role of phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods A549 cells in logarithmic growth phase were divided into three groups:control group,experiment group A and experiment group B.The control group were added serum-free RPMI-1640 medium,the Lactin group A were added 10μg/mL endogenin to the cells for 60 min,and the Lactin group B were added 20μg/mL endogenin to the cells for 60 min.CCK-8 method were used to detect cell proliferation,double staining method were used to detect cell apoptosis,Transwell experiment were used to detect cell invasion,and Western Blotting method were used to detect PI3K/AKT protein expression.Results At 24 h and 36 h after treatment,the cell proliferation index and the number of transmembrane cells in the visfatin B group and visfatin A group were higher than those of the control group,and the visfatin B group was also significantly higher than the visfatin A group,the differences were statistically significant(P<0.05).The apoptosis rate of the visfatin B group and the visfatin A group was significantly lower than that of the control group,and the visfatin B group was also significantly lower than that of the visfatin A group,the differences were statistically significant(P<0.05).The relative expression levels of PI3K and AKT protein in the visfatin B group and the visfatin A group were significantly higher than those of the control group,and the visfatin B group was also significantly higher than that of the visfatin A group,the differences were statistically significant(P<0.05).Conclusion The application of Lactin in lung cancer cells can promote apoptosis,inhibit cell proliferation and clarity,and also can inhibit the activation of PI3K/AKT signaling pathway,thereby exerting tumor suppressing effect.