摘要
目的分析不同产地茯苓的成分差异,为茯苓的产地溯源研究提供参考。方法采用Sepax HP-C_(18)柱(4.6 mm×150 mm,5μm),以0.5%乙酸水溶液(A)-乙腈(B)为流动相进行梯度洗脱,建立特征图谱,结合正交偏最小二乘法判别分析(OPLS-DA)筛选不同产地茯苓的差异成分。结果建立了茯苓的特征图谱,标定并归属21个共有峰和9个非共有峰;通过OPLS-DA共筛选出7种差异成分,包括3种共有成分和4种非共有成分。结论通过OPLS-DA分析茯苓特征图谱的差异,并筛选出7种差异成分可用于茯苓产地溯源研究。
OBJECTIVE To analyze the compositional difference of Poria cocosand provide a reference for the origin traceability of Poria cocos.METHODS Sepax HP-C_(18)(4.6 mm×150 mm,5μm)column was applied to the establishment of the fingerprint of Poria cocos,with acetonitrile-water(0.5%acetic acid)as mobile phase.Then,screen out the different components inPoria cocos from different habitatsby orthogonal partial least squares discriminant analysis(OPLS-DA).RESULTS A total of 21 common peaks and 9non-common peaks were identified inthe fingerprint of Poria cocos.Through OPLS-DA screened out 7 different components,including 3 common components and 4 non-common components.CONCLUSION OPLS-DA was used to analyze the differences in the fingerprint,and screened out 7 different components that could be used for the traceability of Poria cocos.
作者
孙瑞
李任时
余伯阳
SUN Rui;LI Ren-shi;YU Bo-yang(Department of Resources Science of Traditional Chinese Medicines,China Pharmaceutical University,Nanjing 210009,China;Jiangsu Key Laboratory of TCM Evaluation and Translational Research,China Pharmaceutical University,Nanjing 210009,China;Research Center for Traceability and Standardization of TCMs,China Pharmaceutical University,Nanjing 210009,China)
出处
《海峡药学》
2021年第9期18-22,共5页
Strait Pharmaceutical Journal
