基于数据挖掘和网络药理学探讨中医药治疗急性胰腺炎的用药规律及潜在机制

Explore the Medication Rules and Potential Mechanism of Chinese Medicine in the Treatment of Acute Pancreatitis Based on Data Mining and Network Pharmacology

目的基于数据挖掘和网络药理学探讨中医药治疗急性胰腺炎的用药规律,探讨核心药对治疗急性胰腺炎的作用机制。方法检索2020年1月1日至2020年12月31日在广州中医药大学第一附属医院住院且第一诊断为急性胰腺炎的患者中药处方,建立处方数据库,分析用药频次、关联规则及聚类分析。利用相关数据库及软件分析药对-疾病共同靶点、相互作用关系及富集通路。结果筛选出中药处方238首,共涉及164味中药,频次最高的药物为柴胡,得到置信度最高的核心药对柴胡-黄芩-大黄,检索柴胡、黄芩、大黄共得到49种活性成分及185个靶基因,检索急性胰腺炎的靶点为576个,药对-疾病共同靶点为71个。根据Degree值得出核心靶点为AKT1、TNF、IL-6、MAPK1等。通过GO和KEGG富集分析得出核心药对治疗急性胰腺炎的作用机制主要涉及凋亡过程的负调控、RNA聚合酶Ⅱ启动子转录的正调控、PI3K-Akt信号通路等。结论柴胡-黄芩-大黄通过多靶点、多途径发挥治疗急性胰腺炎的作用,为临床中医药治疗急性胰腺炎提供了新思路。

Objective To explore the medication rule of traditional Chinese medicine in the treatment of acute pancreatitis based on data mining and network pharmacology,and to explore the mechanism of core medicine in the treatment of acute pancreatitis.Methods The prescriptions of patients with acute pancreatitis who were hospitalized in the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine from January 1,2020 to December 31,2020 were searched,and the prescription database was established to analyze the medication frequency,association rules and cluster analysis.The drug pair common target,interaction relationship and enrichment pathways were analyzed by relevant databases and software.Results A total of 238 Chinese medicine prescriptions were screened,involving 164 traditional Chinese medicines.The most frequent drug was bupleurum,and the core drug pair Radix Bupleuri-Scutellariae-Rhubarb with the highest confidence was obtained.49 active components and 185 target genes were obtained by searching Radix Bupleuri,Scutellaria and Rhubarb.576 targets were searched for acute pancreatitis,and 71 common targets were searched for drug pair disease.According to the degree value,the core targets were AKT1,TNF,IL-6,MAPK1,etc.Through Go and KEGG enrichment analysis,we found that the mechanism of core drugs in the treatment of acute pancreatitis is mainly related to the negative regulation of apoptotic process,positive regulation of transcription from RNA polymerase II promoter,and PI3K-Akt signaling pathway.Conclusion Radix Bupleuri-Scutellariae-Rhubarb plays a role in the treatment of acute pancreatitis through multiple targets and pathways,which provides a new idea for the clinical treatment of acute pancreatitis with traditional Chinese medicine.