基于金纳米簇-牛血清白蛋白无酶无标记荧光探针测定芦丁的研究

Detection of Rutin Using an Enzyme-free and Label-free Fluorescent Probe Based on Gold Nanoclusters-Bovine Serum Albumin

以牛血清白蛋白(BSA)为模板,制备了金纳米簇-牛血清白蛋白(AuNCs-BSA)荧光探针,构建了一种无酶无标记检测芦丁的荧光传感分析方法。采用透射电镜(TEM)、紫外-可见光谱(UV-Vis)、荧光光谱(FL)表征了AuNCs-BSA的形貌和光学特性。实验结果表明,当存在芦丁时,由于BSA与芦丁的亲和力强于AuNCs,AuNCs从BSA中释放出来,导致体系的荧光猝灭。在优化实验条件下,AuNCs-BSA荧光探针对芦丁检测的线性范围为0~60μmol/L,检出限(S/N=3)为0.63μmol/L。实际样品在低、中、高3个水平下的加标回收率为99.0%~103%,相对标准偏差小于3%。该荧光探针制备简单、无需任何标记、灵敏度高,为实际样品中芦丁含量的测定提供了一种简单、可靠、有效的方法。

An enzyme-free and label-free fluorescence sensing method for rutin detection was devel⁃oped based on gold nanoclusters-bovine serum albumin(AuBCs-BSA)fluorescent probe prepared with bovine serum albumin(BSA)as template.The morphology and optical property of the prepared AuNCs-BSA were characterized by transmission electron microscopy(TEM),ultraviolet-visible spectroscopy(UV-Vis)and fluorescent spectroscopy(FL).The experimental results showed that fluorescence of AuNCs-BSA system was quenched in the presence of rutin as the affinity between BSA and rutin was stronger than that between BSA and AuNCs,resulting in the release of AuNCs from BSA.Under the optimized experimental conditions,the linear range of AuNCs-BSA fluores⁃cent probe for rutin detection was 0-60μmol/L with a detection limit(S/N=3)of 0.63μmol/L.The spiked recoveries of real sample were 99.0%-103%with relative standard deviations less than 3%.The developed fluorescent probe has the advantages of simple preparation,no labeling and high sensitivity,and provides a simple,reliable and effective method for the determination of rutin in practical samples.