高通量测序分析根结线虫对马铃薯根际土壤真菌群落多样性的影响

High-Throughput Sequencing on Fungal Diversity in Potato Rhizosphere Soil Infested by Root-knot Nematodes

【目的】分析根结线虫对马铃薯根际土壤真菌群落多样性的影响。【方法】通过高通量测序技术、土壤性质测定及生物信息学技术分析感染根结线虫马铃薯和健康马铃薯根际土壤真菌群落多样性差异。【结果】从6个土壤样品中共获得3界12门34纲77目171科332属526种的土壤真菌。对物种多样性指数分析后,发现感染根结线虫马铃薯病株与健康马铃薯植株的根际土壤真菌群落多样性和土壤性质均存在显著性差异。对6个样品进行物种组成分析后,在门水平中,感病植株根际土壤真菌Basidiomycota丰度较健康植株根际土壤真菌丰度高,Ascomycota丰度低于健康植株根际土壤;在科水平中,病株根际土壤丰度较高的菌群有Nectriaceae和Trimorphomycetaceae;在属水平中,健康植株根际土壤丰度较高的菌群为镰刀菌属Fusarium、被孢霉属Mortierella和未知分类毛壳菌科属unclassified_f_Chaetomiaceae。【结论】感染根结线虫的马铃薯与健康马铃薯根际土壤真菌群落丰度在门、科和属水平上均存在差异,为进一步研究马铃薯根结线虫的发生和防治提供了新的思路。

【Objective】Diversity of the rhizosphere soil fungal community in potato field as affected by the presence of rootknot nematodes was investigated using high-throughput sequencing technique.【Methods】The properties and bioinformatics of rhizosphere soils surrounding healthy and infested potato plants were compared to analyze the possible effects of differential fungal community on the occurrence and control of the disease.【Results】There were 526 fungi species belonging to 332 genera,171 families,77 orders,34 classes,12 phyla,and 3 kingdoms identified from the 6 field specimens.Significantly differentiations on the fungal community diversity and properties of the rhizosphere soils on the healthy and infested potato lots were found.In the infected areas,Basidiomycota was the more abundant phylum,but Ascomycota less,than on the healthy lots.At family level,Nectriaceae and Trimorphomycetaceae were more abundantly found in the soil of diseased plants,while at genus level,Fusarium,Mortierella,and some unclassified Chaetomiaceae more richly in the soil of healthy plants.【Conclusion】Significant differences in the abundance of fungi in the rhizosphere soils of potatoes infested by the root-knot nematodes and the healthy counterparts were found at phylum,family,and genus levels.The information would aid further study on the occurrence and control of potato root-knot nematodes.