基于高通量测序的中国水仙根际土壤微生物群落组成

High-throughput Sequencing on Microbial Community in Rhizosphere Soil of Chinese Narcissus

【目的】明确中国水仙植株根际土壤微生物群落的组成特征。【方法】采用Illumina Miseq高通量测序技术对水仙根际土壤微生物样品的保守基因区进行测序及生物信息学分析,阐明中国水仙根际土壤的细菌、真菌和古菌群落结构组成,并对水仙根际土壤微生物的优势菌属进行深入分析。【结果】共获得优化序列175840条,基于97%序列相似度,聚类为2680个OTUs。优势细菌类群是绿弯菌门Chloroflexi(30.86%)和变形菌门Proteobacteria(20.67%),真菌以子囊菌门Ascomycota(84.94%)为主,属水平上球毛壳菌Chaetomium globosum(28.15%)和散子囊菌Eurotiales(25.01%)占较高比例。古菌类群主要为奇古菌门Thaumarchaeota(51.40%)、深古菌门Bathyarchaeota(25.98%)和广古菌门Euryarchaeota(20.65%)。其中,来自奇古菌门的SCG类群(25.67%)和嗜酸性氨氧化古菌Candidatus_Nitrosotalea(12.93%)占较高比例。【结论】中国水仙根际土壤微域具有丰富多样的微生物类群,这对于开发和利用水仙根际土壤微生物资源具有重要意义。

【Objective】The microbial community in rhizosphere soils of Chinese narcissuses was analyzed.【Method】The Illumina Miseq high-throughput sequencing technology was used to obtain the relevant conserved gene regions of the microbes in soil specimens from Chinese narcissus planting lots.The community structures and distributions of dominant species of bacteria,fungi,and archaea in the rhizosphere soils were analyzed.【Result】In total,175840 optimized sequences were obtained and clustered from the specimens into 2680 representative OTUs with a 97%similarity.The dominant bacteria were Chloroflexi(30.86%)and Proteobacteria(20.67%).Among the fungi,Ascomycota(84.94%)significantly overshadowed the others,with Chaetomium globosum(28.15%)and Ascomycetes(25.01%)accounted for the greater proportions.On archaea,Thaumarchaeota(51.40%),Bathyarchaeota(25.98%),and Euryarchaeota(20.65%)were the major phyla that had 25.67%SCG and 12.93%acidophilic ammonia oxidizing members.【Conclusion】The rhizosphere soils of Chinese narcissuses harbored diverse and rich microbial species.The information obtained would aid the development and utilization of the natural resources.