丁酸钠对副溶血性弧菌毒性的影响及机制研究

Effect and mechanisms of sodium butyrate on the virulence of Vibrio parahaemolyticus

目的探究丁酸钠对副溶血性弧菌毒性的影响及机制。方法通过测定丁酸钠对副溶血性弧菌生物膜形成能力及黏附宿主细胞能力,评估其对副溶血性弧菌毒性的影响;通过傅里叶变换红外光谱法(Fourier transform infrared spectroscopy,FT-IR)和拉曼光谱法检测胞外成分变化;逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-qPCR)测定毒力基因转录水平变化。结果亚致死浓度(sub-inhibitory concentrations,SICs)的丁酸钠基本不影响副溶血性弧菌生长,但对生物膜形成有显著抑制作用;丁酸钠处理后,副溶血性弧菌对Caco-2细胞的黏附率分别降低28%、41%和61%;FT-IR和拉曼光谱结果表明丁酸钠能影响副溶血性弧菌脂肪酸及蛋白质疏水性以及降低核酸、碳水化合物和蛋白质的峰面积;RT-qPCR分析显示0.500 mg/mL丁酸钠可显著下调与副溶血性弧菌毒力相关基因的转录表达(P<0.01)。结论丁酸钠具有一定的抑制副溶血性弧菌毒性的能力,有用于预防及控制副溶血性弧菌造成的食源性疾病的潜力。

Objective To explore the effect and mechanisms of sodium butyrate on the virulence of Vibrio parahaemolyticus.Methods The effect of sodium butyrate on the virulence of Vibrio parahaemolyticus was evaluated by measuring its biofilm formation ability and host cell adhesion ability;Fourier transform infrared spectroscopy(FT-IR)and Raman spectroscopy were used to detect the changes in extracellular components,and the changes in virulence genes expression were detected by reverse transcription-polymerase chain reaction(RT-qPCR).Results Sodium butyrate at sub-inhibitory concentrations(SICs)had minimal effect on the growth of Vibrio parahaemolyticus,but had significant inhibitory effects on bacterial biofilm formation;the adhesion rates of Vibrio parahaemolyticus to Caco-2 cells were decreased by 28%,41%and 61%respectively with sodium butyrate treatment under different concentrations.The results of FT-IR and Raman spectroscopy suggested that sodium butyrate could affect the fatty acid,protein,hydrophobicity of Vibrio parahaemolyticus,and reduce the peak area of nucleic acid,carbohydrate,and protein;RT-qPCR analysis revealed that 0.500 mg/mL sodium butyrate significantly down-regulated transcriptional expression of genes associated with virulence(P<0.01).Conclusion Sodium butyrate has the ability to inhibit virulence of Vibrio parahaemolyticus and has potential to be used in the prevention and control of food-borne diseases caused by Vibrio parahaemolyticus.