QuEChERS结合高效液相色谱-串联质谱法测定大白菜中10种喹诺酮类药物残留

Determination of 10 kinds of quinolones residues in cabbage by QuEChERS coupled with high performance liquid chromatography-tandem mass spectrometry

目的建立QuEChERS结合高效液相色谱-串联质谱法测定大白菜中10种喹诺酮类药物残留的分析方法。方法大白菜样品经提取,采用QuEChERS净化方法,以诺氟沙星-D5为内标,用高效液相色谱-串联质谱法测定,采用正离子电喷雾,多反应监测模式,内标法定量分析10种喹诺酮类药物残留的含量。结果10种喹诺酮类在5~100 ng/mL的质量浓度范围内线性良好,检出限为0.1~0.3μg/kg,定量限为0.3~1.0μg/kg,平均回收率为76.0%~107.0%,相对标准偏差为1.5%~8.1%。结论该方法准确、可靠,适用于大白菜中喹诺酮类药物残留的检测。

Objective To establish a method for the determination of 10 kinds of quinolones residues in cabbage by QuEChERS coupled with high performance liquid chromatography-tandem mass spectrometry.Methods The cabbage samples were extracted,using the QuEChERS purification method and norfloxacin D5 as internal standard,and quantitatively determined by high performance liquid chromatography-tandem mass spectrometry.The content of 10 kinds of quinolones residues were quantitatively analyzed by positive ion electrospray and multi-reaction monitoring mode.Results The 10 kinds of quinolones showed good linearity in the concentration range of 5-100 ng/mL,the limits of detection were 0.1-0.3μg/kg,the limits of quantitation were 0.3-1.0μg/kg,the average recoveries were 76.0%-107.0%,and the relative standard deviations were 1.5%-8.1%.Conclusion This method is accurate,reliable and suitable for the determination of quinolones residues in cabbage.