摘要
该文结合金属有机框架(MOFs)材料的良好固载性能[1]和金纳米颗粒(Au NPs)优秀的催化活性[2],合成ZIF-8-鲁米诺-金纳米颗粒复合物(ZIF-8-Lu-Au NPs),并将ZIF-8-Lu-Au NPs和壳聚糖(CS)层层组装修饰于玻碳电极(GCE)表面构建ECL传感器,用于对多巴胺(DA)的灵敏检测。采用循环伏安法对电极的修饰过程进行验证,通过电化学发光(ECL)技术对制备的传感器的性能进行表征。结果表明,该ECL生物传感器对DA有良好的ECL响应,其线性范围为1.0×10^(-8)~1.0×10^(-5) mol/L,检出限为4.47×10^(-10) mol/L。该方法借助ZIF-8-Lu-Au NPs优异而稳定的ECL性能和DA对ECL信号的淬灭作用,实现了对多巴胺的灵敏检测,该方法简单、快速、特异性和灵敏度高,在临床诊断和超灵敏生物分析检测方面具有较强应用潜力。
Herein,based on the outstanding immobilization performance of metal organic frameworks(MOFs)compound and the excellent catalytic activity of gold nanoparticles(Au NPs),an electrochemiluminescent(ECL)sensitive biosensor for the determination of dopamine(DA)was constructed with the combination of ZIF-8,luminol,and Au NPs(ZIF-8-Lu-Au NPs).At first,the MOF-Lu-Au NPs composites and chitosan were immobilized on the glassy carbon electrode(GCE)surface alternatively.Then,the electrode was inserted into phosphate buffer saline(PBS)containing a certain amount of DA and 4.0 mmol/L of hydrogen peroxide.In the experiment,the preparation process of the ECL biosensor was characterized by the cyclic voltammetry and the performance of the biosensor was characterized by electrochemiluminescence.The results showed that the biosensor presented high sensitivity,with a linear range of 1.0×10^(-8)~1.0×10^(-5) mol/L and a detection limit of 4.47×10^(-10) mol/L.By virtue of the excellent and stable ECL performance of ZIF-8-Lu-Au NPs and the quenching effect of DA on ECL signal,this method realized the sensitive detection of dopamine.It has the advantages of high specificity and sensitivity,fast analysis speed,simplicity and convenience,and convenient,and was expected to be applied to clinical disease diagnosis and biological analysis detection.
作者
苏美玲
刘伟
柴雅琴
Su Mei-ling;Liu Wei;Chai Ya-qin(College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)
出处
《化学传感器》
CAS
2019年第4期33-38,共6页
Chemical Sensors
基金
国家级大学生创新训练项目(202010635035)。
