参与番茄叶形发育的TCP转录因子的表达及生物信息分析

Expression of SlTCPs Transcription Factors involved in Tomato Leaf Shape Development and Bioinformatic Analysis

TCP是植物特有的一类转录因子,在植物的生长发育过程中起着重要作用。本试验选取18个在番茄叶片组织中高表达的TCP转录因子,检测其在叶片不同发育时期的表达水平,筛选出与叶片发育进程相关的SlTCP2、SlTCP4、SlTCP8、SlTCP11、SlTCP14、SlTCP15、SlTCP19、SlTCP22、SlTCP24、SlTCP25等10个TCP家族成员。对其进行生物信息分析,结果表明这10个SlTCP成员分布在8条不同的染色体上,它们的碱基、氨基酸长度以及分子量大小相差较大,均为不稳定的亲水性蛋白;SlTCPs的蛋白结构和成分分析表明,中间约50 aa长度的氨基酸序列保守性相对较高且丝氨酸占比较多;SlTCPs蛋白的亚细胞定位预测均在细胞核上。以上SlTCPs蛋白均含有TCP家族典型的保守域,其保守基序的分类与系统进化树的分支一致。组织表达分析结果表明,SlTCP11、SlTCP15、SlTCP24在番茄叶片发育各个时期的不同组织器官中,均有相对较高的表达水平。对番茄典型叶形突变体LA2922、LA2532及其对应的野生型进行qRT-PCR,结果表明SlTCP2、SlTCP11、SlTCP14、SlTCP15、SlTCP19、SlTCP24在LA2922和LA2532叶片中的表达水平呈相反趋势。结合生物信息分析结果,明确了SlTCP2、SlTCP11、SlTCP14、SlTCP15、SlTCP19、SlTCP24可能在番茄叶形发育中起关键作用,为进一步探究番茄叶片生长发育的分子机制奠定了基础。

TCP is a class of transcription factors unique to plants and plays an important role in plant growth and development.Selecting 18 TCP members highly expressed in tomato leaf tissue,this study detected their expression levels at different development stages;and screened out 10 TCP family members related to leaf blades development process,including SlTCP2,SlTCP4,SlTCP8,SlTCP11,SlTCP14,SlTCP15,SlTCP19,SlTCP22,SlTCP24,SlTCP25,etc..Then bioinformatic analysis was performed on them.The results indicated that these 10 SlTCPs members were distributed in 8 different chromosomal locations.There were relatively larger differences in their length of basic group,amino acid and molecular weight.And all of the TCPs were unstable hydrophilic proteins.Analysis of SITCPs protein structure and element indicated that in center about 50aa length amino acid sequences were relatively well conserved.Moreover,serine took relatively larger proportion.The site prediction of SlTCPs protein subcellular fraction were all localized on the nucleus.The above mentioned SlTCPs protein all contained the conserved domains of typical TCP family.Its classification of conserved motif was consistent with the branching of phylogenetic tree.Results of tissue expression analysis showed that SlTCP11,SlTCP15 and SlTCP24 all had relatively higher expression levels in different tissues and organs at each stage.Using typical tomato leaf shape mutant materials LA2922 and LA2532 and their corresponding wild types,this experiment conducted qRT-PCR.The results indicated that the expression levels of SlTCP2,SlTCP11,SlTCP14,SlTCP15,SlTCP19 and SlTCP24 in leaf blades of LA2922 and LA2532 were in the opposit direction.Combining with the results of bioinformation analysis,it was specified that SlTCP2,SlTCP11,SlTCP14,SlTCP15,SlTCP19,and SlTCP24 might play a key role in the development of tomato leaf shape.This has laid foundation for further explorating the molecular mechanism of tomato leaf blades growth and development.