注射波生坦阻滞内皮素受体减轻大鼠口腔癌性疼痛

Injection of bosentan alleviates oral cancer pain by blocking endothelin receptor

目的研究阻滞内皮素受体对大鼠口腔癌所引起的疼痛的影响。方法Wistar大鼠48只分为4组:假手术组、口腔癌模型+生理盐水组、口腔癌模型+三叉神经节注射药物组、口腔癌模型+舌部注射药物组,造模前测定4组大鼠口面部基础机械痛阈。口腔癌模型大鼠右侧舌部注射Walker256肿瘤细胞进行造模,每日监控体重变化。造模第8天测定口面部机械痛阈,并观察10 min面部自发痛行为时长;造模后第9天注射内皮素受体拮抗剂波生坦,给药后1 h测定口面部机械痛阈,给药后90 min观察10 min内面部自发痛行为时长。结果采用舌部注射Walker256肿瘤细胞法成功制备大鼠口腔癌模型;荷瘤大鼠体重显著下降(P<0.01),口面部机械痛阈显著下降(P<0.01),10 min内面部自发痛行为时长显著增加(P<0.05)。三叉神经节/舌部注射波生坦后1 h,荷瘤大鼠的机械痛阈显著上升(P<0.01),注射后90 min,10 min内面部自发痛行为时长明显减少(P<0.01)。结论通过注射波生坦阻滞内皮素受体在一定时间内对大鼠口腔癌性疼痛具有减轻作用。

Objective To investigate the effect of blocking endothelin receptors on the pain of oral cancer in rats.Methods A total of 48 Wistar rats were selected and divided into four groups:a sham group,a tumor model+normal saline group,a tumor model+trigeminal ganglion injection group,and a tumor model+tongue injection group.Before modeling,basic oral and facial mechanical pain thresholds in the four groups were measured.Walker 256 tumor cells were injected into the right tongue of rats to establish the model,and weight changes were daily monitored.On day 8 after modeling,the oral and facial mechanical pain threshold was measured,and the duration of spontaneous facial pain within 10 min was observed.On day 9 after modeling,bosentan,an endothelin receptor antagonist,was injected.Then,1 h after injection,the mechanical pain threshold in the oral and facial region of rats was measured and 90 min after injection,the duration of spontaneous pain behavior in the facial region of rats within 10 min was observed.Results The oral cancer model of rats was successfully established by injecting Walker 256 tumor cells.The weight of tumor bearing rats significantly decreased(P<0.01),the oral and facial mechanical pain threshold of the oral cancer rats dramatically decreased(P<0.01),and the duration of spontaneous pain behavior in the facial region within 10 min substantially increased(P<0.05).Then,1 h after injection of bosentan into the trigeminal ganglion/tongue,the oral and facial mechanical pain threshold of the oral cancer rats significantly increased(P<0.01)and 90 min after injection,the duration of spontaneous facial pain behavior within 10 min significantly decreased(P<0.01).Conclusions Injection of bosentan can block endothelin receptor and relieve the pain of oral cancer in rats within a certain period of time.