ACAP1在卵巢浆液性囊腺癌中的表达及其意义

Expression and significance of ACAP1 in ovarian serous carcinoma

目的检测ACAP1在卵巢浆液性囊腺癌中的表达情况,明确其与卵巢浆液性囊腺癌发生发展的关系。方法通过Oncomine网站分析ACAP1 mRNA表达水平与卵巢癌发生的相关性,通过cBioportal网站分析卵巢癌组织中ACAP1基因突变的类型,通过GeneMANIA网站分析ACAP1相关信号通路以及相互作用的蛋白。采用免疫组化方法检测ACAP1在卵巢浆液性囊腺癌中的表达情况。结果ACAP1 mRNA在卵巢癌组织中的表达水平高于正常卵巢组织;ACAP家族在卵巢癌组织中存在多种基因突变。应用GeneMANIA网站构建ACAP家族基因互作网络,其中与ACAP家族关系最密切的5个基因为GULP1、ARF6、RAB11FIP3、SLC2A4、VAMP3;ACAP1在卵巢浆液性囊腺癌组织中的表达水平高于正常卵巢组织(P<0.001);ACAP1在卵巢癌原发灶中的高表达与患者的不良预后相关(P=0.037);ACAP1在卵巢癌转移灶中的表达高于卵巢癌原发灶(P<0.001)。结论ACAP1在卵巢浆液性囊腺癌中高表达,在卵巢癌转移灶中的表达高于原发灶。ACAP1在原发灶中的高表达与患者的不良预后相关。ACAP1可能与卵巢浆液性囊腺癌的发生发展有关,可能成为卵巢癌诊治的新靶点。

Objective To detect the expression of ADP-ribosylation factor GTPase-activating protein with coiled-coil,ankyrin repeat and pleckstrin homology domains 1(ACAP1)in ovarian serous carcinoma,and to determine the relationship between ACAP1 and the occurrence and development of ovarian serous carcinoma.Methods The Oncomine database was used to analyze the mRNA expression of ACAP1 in ovarian cancer.The cBioportal database was used to analyze genetic variations in ACAPs.The GeneMANIA database was used to analyze the gene-gene interaction network of ACAPs.The expression of ACAP1 in tissues was studied by immunohistochemistry.Results The mRNA expression of ACAP1 in ovarian cancer tissues was higher than that in normal ovarian tissues.Many types of gene mutations were detected in the ACAP family.Using the GeneMANIA database,we constructed the gene-gene interaction network of ACAPs;the five genes most closely related to the ACAP family were GULP1,ARF6,RAB11FIP3,SLC2A4,and VAMP3.The expression of ACAP1 in ovarian serous carcinoma tissue was higher than that in normal ovarian tissue(P<0.001).The high expression of ACAP1 in the primary tumor was significantly associated with poor prognosis(P=0.037).The expression of ACAP1 in ovarian cancer metastases was higher than that in the primary ovarian cancer(P<0.001).Conclusion ACAP1 may be related to the occurrence and development of ovarian serous carcinoma.It has potential as a novel diagnostic and therapeutic target for ovarian cancer.