基于树状PAMAM修饰的PDMS微流控芯片分离检测两种蛋白质

Separation and Detection of Two Proteins Using Dendritic PAMAM Modified PDMS Microfluidic Chip

为提高聚二甲基硅氧烷(PDMS)微流体芯片的亲水性,抑制其对蛋白质的吸附,利用树状PAMAM通过物理涂覆的手段对其微通道的表面进行了修饰。对修饰后PDMS微流控芯片的亲水性和电渗流进行了考察,同时利用修饰后芯片对溶菌酶,核糖核酸酶A两种蛋白质进行了分离检测。结果表明,修饰后的PDMS微流控芯片微通道表面形成了一层均匀、稳定的亲水性涂层,有效抑制了对蛋白质的吸附,成功地对溶菌酶和核糖核酸酶A实现了分离,柱效分别达到6.93×10^(4) plates/m和7.92×10^(4) plates/m,同时修饰后的芯片具有良好的重现性和较长的使用寿命。

In order to improve the hydrophilicity of the polydimethylsiloxane(PDMS)microfluidic chip and inhibit the adsorption of proteins on it,the surface of its micro-channel was modified by using dendrimeric PAMAM via physical coating.The hydrophilicity and electroosmotic flow of the modified PDMS microfluidic chip were investigated,and the lysozyme and ribonuclease A were separated and detected using the modified chip.The results showed that the surface of the modified chip possessed a uniform and stable hydrophilic coating,which effectively inhibited the adsorption of proteins,and lysozyme and ribonuclease A was successfully separated with 6.93×10^(4) plates/m and 7.92×10^(4) plates/m respectively.In addition,the modified chip has good reproducibility and long service life.