Effects of Rhynchophylline on Learning and Memory of Alzheimer's Disease Zebrafish

[Objectives]To investigate the effect of rhynchophylline on the behavior of Alzheimer's disease zebrafish induced by aluminum trichloride(AlCl3),to provide theoretical basis for the development and application of rhynchophylline in the field of Alzheimer's disease.[Methods]The video recording was made on zebrafish before training,so as to judge whether zebrafish training was successful or not.After training zebrafish for 7 d,60 zebrafish were randomly divided into 6 groups:blank group,model group,positive group,low-dose rhynchophylline group,medium-dose rhynchophylline group and high-dose rhynchophylline group.The blank group was video-recorded,while the model group,positive group,low-dose group,medium-dose group and high-dose group were exposed to AlCl3 for modelling.After the end,the model group was video-recorded,and the other groups were given drug intervention.Both the positive group and the rhynchophylline group were given drug for 6 d,and finally all the groups were video-recorded.After all the videos were finished,the behavioral analysis was carried out by using the behavioral record analysis software smart3.0,and the conclusion was drawn by analyzing the data.[Results]The data of length of stay of zebrafish in red short arm area of 6 groups were compared and analyzed:there was a significant difference between blank group and model group(P<0.05),and there was a significant difference between model group and positive group,medium-dose group or high-dose group(P<0.05).The percentage of time spent in the red short arm area of 6 groups of zebrafish was compared and analyzed:there was a significant difference between blank group and model group(P<0.05),and there was a significant difference between model group and positive group,medium-dose group or high-dose group(P<0.05).The data of swimming distance of 6 groups of zebrafish in red short arm area were compared and analyzed:there was no significant difference between the blank group and the model group(P>0.05),but there was only significant difference between the model group and the high-dose group(P<0.05).The percentage of swimming distance of zebrafish in the red short arm area of the six groups was compared and analyzed:there was a significant difference between the blank group and the model group(P<0.05),and there were significant differences between model group and positive group,medium-dose group or high-dose group(P<0.05).[Conclusions]Rhynchophylline can improve the behavior of Alzheimer's disease zebrafish.