骨肉瘤组织ACTN4和CD133的表达及其影响因素

Expression and interfering factors of ACTN4 and CD133 in osteosarcoma

目的观察α-辅肌动蛋白(α-actinin-4,ACTN4)和CD133在骨肉瘤组织中的表达情况,并探讨影响两者表达的相关因素。方法收集2015年1月至2018年1月经病理确诊为骨肉瘤的50例患者临床病理资料,应用免疫组化检测ACTN4和CD133的蛋白表达情况,分析各因素不同水平间ACTN4和CD133蛋白表达的差异。结果ACTN4在骨肉瘤组织中阳性率为58%,CD133在骨肉瘤组织中阳性率为54%,两者阳性率均高于其在骨软骨瘤组织中的表达,差异均具有统计学意义(P<0.05)。不同Enneking外科分期、肺转移之间的ACTN4表达差异有统计学意义(P<0.05)。不同Enneking外科分期、局部复发和肺转移之间的CD133表达差异有统计学意义(P<0.05)。结论ACTN4和CD133在骨肉瘤发生、发展中具有重要作用。

Objective To observe the expression of α-actinin-4(ACTN4)and CD133 in osteosarcoma and to explore the related factors affecting their expression.Methods The clinicopathological data of 50 patients with osteosarcoma diagnosed by pathology from January 2015 to January 2018 were collected.The protein expression of ACTN4 and CD133 was detected by immunohistochemistry,and the differences of protein expression among different levels of various factors were further analyzed.Results The positive rates of ACTN4 and CD133 in osteosarcoma were 58% and 54%,respectively.Both positive rates were higher than their expression in osteochondroma.There were significant differences in the expression of ACTN4 between different Enneking surgical stages and lung metastasis(P<0.05).There were significant differences in the expression of CD133 between different Enneking surgical stages,local recurrence and lung metastasis(P<0.05).Conclusion ACTN4 and CD133 play an important role in the occurrence and development of osteosarcoma.