不同程度非酒精性脂肪性肝病患者血清炎症因子及免疫因子表达的研究

Study on the expression of serum inflammatory factors and immune factors in patients with different degrees of nonalcoholic fatty liver disease

目的:探讨不同程度非酒精性脂肪性肝病(NAFLD)患者血清炎症因子及免疫因子表达水平及意义。方法:选取2019年1月至2020年1月NAFLD患者80例(NAFLD组),其中轻度患者25例,中度患者31例,重度患者24例;同时选取健康体检者80例作为对照组;检测各组患者血清白细胞介素-17(IL-17)、白细胞介素-18(IL-18)、白细胞介素-22(IL-22)、肿瘤坏死因子-α(TNF-α)、CD4^(+)、CD8^(+)和CD4^(+)CD25^(+)。结果:NAFLD组血清IL-17、IL-18、IL-22、TNF-和CD4^(+)表达水平分别为(31.15±8.44)pg/ml、(85.50±18.81)pg/ml、(58.84±11.78)pg/ml、(3.94±0.90)pg/ml和(37.40±2.15)%,明显高于对照组(P<0.05),CD8^(+)和CD4^(+)CD25^(+)表达水平分别为(22.84±2.09)%和(8.20±1.84)%,明显低于对照组(P<0.05);NAFLD组重度患者血清IL-17、IL-18、IL-22、TNF-α和CD4^(+)表达水平明显高于轻度和中度患者(P<0.05),而CD8^(+)和CD4^(+)CD25^(+)表达水平明显低于轻度和中度患者(P<0.05);IL-17、IL-18、IL-22、TNF-α、CD4^(+)表达水平与严重程度呈正相关(rs=0.565、0.532、0.311、0.304和0.321,P<0.05),CD8^(+)和CD4^(+)CD25^(+)与严重程度呈负相关(rs=-0.511和-0.503,P<0.05)。结论:NAFLD患者血清炎症因子及免疫指标表达异常,与患者严重程度存在一定相关性。

Objective:To investigate the expression and significance of serum inflammatory factors and immune factors in patients with nonalcoholic fatty liver disease(NAFLD).Methods:A total of 80 patients with NAFLD(NAFLD group)from January 2019 to January 2020 were selected,including 25 mild patients,31 moderate patients and 24 severe patients,and 80 healthy patients were selected as the control group;the serum interleukin-17(IL-17),interleukin 18(IL-18),interleukin22(IL-22),tumor necrosis factor-α(TNF-α),CD4^(+),CD8^(+)and CD4^(+)CD25^(+)were detected.Results:The serum IL-17,IL-18,IL-22,TNF-αand CD4^(+)in the NAFLD group were(31.15±8.44)pg/ml,(85.50±18.81)pg/ml,(58.84±11.78)pg/ml,(3.94±0.90)pg/ml and(37.40±2.15)%,which were significantly higher than those in the control group(P<0.05),while CD8^(+)and CD4^(+)CD25^(+)were(22.84±2.09)% and(8.20±1.84)%,in the which were significantly lower than the control group(P<0.05);The serum IL-17,IL-18,IL-22,TNF-αand CD4^(+)in severe patients in AFLD group were significantly higher than those in mild and moderate patients(P<0.05),while CD8^(+)and CD4^(+)CD25^(+)were significantly lower than that of mild and moderate patients(P<0.05);IL-17,IL-18,IL-22,TNF-αand CD4^(+)were positively correlated with severity(rs=0.565,0.532,0.311,0.304,and 0.321,P<0.05),while CD8^(+)and CD4^(+)CD25^(+)were negatively correlated with severity(rs=-0.511 and-0.503,P<0.05).Conclusion:The serum inflammatory factors and immune indexes are abnormal in patients with NAFLD,there is a certain correlation with the severity of patients.