SBR反应器中活性污泥胞外聚合物的提取方法比较

Comparison of Extracellular Polymeric Substance Extraction Methods from Activated Sludge in Sequencing Batch Reactor

比较了酸法、碱法、加热法、超声法、超声+阳离子交换树脂(CER)法等5种方法对SBR反应器内活性污泥胞外聚合物(EPS)的提取效果。结果表明,超声+CER法是最有效的提取方法,EPS提取总量达到(129.05±7.76) mg/gVSS,而酸法的EPS提取总量最少。活性污泥EPS的主要成分包括蛋白质(34.69%~63.49%)、腐殖酸(24.93%~48.51%)和多糖(9.65%~18.51%)。碱法、超声法和超声+CER法对蛋白质的提取效率较高,提取量可以达到(56.56±1.49)~(57.30±3.63) mg/gVSS;加热法对腐殖酸的提取量最高,为(55.78±5.08) mg/gVSS。三维荧光光谱分析结果表明,超声+CER法的蛋白质类物质的荧光峰强度最高。傅里叶变换红外光谱分析结果显示,主要的红外吸收峰与蛋白质、多糖等物质的官能团密切相关,这与EPS提取组分含量相吻合。根据EPS总量和各组分提取效果,推荐超声+CER法作为SBR反应器中活性污泥EPS的提取方法。

The extraction efficiency of extracellular polymeric substances(EPS) from activated sludge in the sequencing batch reactor(SBR) by five methods, including acid extraction method, alkali extraction method, heating extraction method, ultrasonic extraction method and ultrasonic/cation exchange resin(CER) extraction method, was compared. The ultrasonic/CER extraction method was the most effective and the extraction amount of EPS reached(129.05±7.76) mg/gVSS. The total amount of EPS extracted by the acid method was the lowest. The main components of the EPS extracted from the activated sludge were protein(34.69%-63.49%), humic acid(24.93%-48.51%) and polysaccharide(9.65%-18.51%). The alkali, ultrasonic and ultrasonic/CER extraction methods had a higher extraction efficiency of the protein, and the extraction amounts were between(56.56±1.49) mg/gVSS and(57.30±3.63) mg/gVSS. The heating extraction method had the highest extraction efficiency of the humic acid with extraction amount of(55.78±5.08) mg/gVSS. The three-dimensional excitation-emission matrix spectrum showed that the fluorescence peak intensity of proteinaceous substances extracted by ultrasonic/CER method was the highest. The Fourier transform infrared spectrum showed that the main absorption peaks were closely related to the functional groups of the protein and polysaccharide, and the results were consistent with the extraction amounts of EPS. The ultrasonic/CER extraction method was recommended to extract EPS from activated sludge in SBRs after comparing the total extraction amounts and components of EPS.