CASP8、Fas基因多态性与汉族非霍奇金淋巴瘤患者预后的关系

Relationships between CASP8,Fas Gene Polymorphisms and the Prognosis of Patients with Non-Hodgkin’s Lymphoma in Han Nationality

目的:探讨胱天蛋白酶8(CASP8)、脂肪酸合成酶(Fas)基因多态性与汉族非霍奇金淋巴瘤患者预后的关系。方法:回顾性分析85例汉族非霍奇金淋巴瘤患者的临床资料,检测CASP8、Fas基因多态性,统计预后情况,对比不同预后患者CASP8、Fas基因多态性,探讨基因多态性与患者预后不良的关系。结果:本组85例患者预后不良发生率为65.88%;预后不良与预后良好患者CASP8、Fas基因多态性均符合Hardy-Weinberg遗传平衡定律;预后不良患者CASP8基因rs 1035142位点GG基因型频率、等位基因G频率,Fas基因rs 1377位点GA基因型频率、等位基因A频率均明显低于预后良好患者(P<0.05),CASP8基因rs 1035142位点GT、TT基因型频率、等位基因T频率,Fas基因rs 1377位点GG基因型频率和等位基因G频率均明显高于预后良好患者(P<0.05);预后不良患者Ann Arbor分期Ⅲ-Ⅳ期、高度恶性占比均明显高于预后良好患者(P<0.05);Logistic多元回归分析显示,Ann Arbor分期Ⅲ-Ⅳ期、中度恶性、高度恶性、CASP8 rs 1035142 GT基因型、CASP8 rs 1035142 TT基因型、Fas rs 1377 GG基因型均为患者预后不良的危险因素(P<0.05)。结论:汉族非霍奇金淋巴瘤患者预后不良发生率较高,Ann Arbor分期Ⅲ-Ⅳ期、中高度恶性、CASP8 rs 1035142 GT基因型、CASP8 rs 1035142 TT基因型、Fas rs 1377 GG基因型均是其预后不良的危险因素。

Objective:To investigate the relationships between caspase-8(CASP8),fatty acid synthetase(Fas)gene polymorphisms and prognosis of non-Hodgkin’s lymphoma patients in Han nationality.Methods:The clinical data of85 patients with non-Hodgkin’s lymphoma were analyzed retrospectively.The polymorphisms of CA SP8 and Fas gene were detected,and prognosis of the patients were analyzed.The polymorphisms of CASP8 and Fas gene in patients with different prognosis were compared,and the relationships between gene polymorphisms and the poor prognosis of the patients were investigated.Results:The incidence rate of poor prognosis of the patients enrolled in the study was65.88%.The polymorphisms of CA SP8 and Fas genes in the patients with poor or good prognosis were in accordance with Hardy Weinberg’s law of genetic balance.The frequencies of GG genotype and G allele at rs 1035142 of CA SP8 gene,GA genotype and A allele at rs 1377 of Fas gene in patients with poor prognosis were lower than those of the patients with good prognosis(P<0.05).The frequencies of GT,TT and T alleles at rs 1035142 of CASP8 gene,GG and G alleles at rs 1377 of Fas gene in patients with poor prognosis were higher than those of the patients with good prognosis(P<0.05).The proportions of Ann Arbor stageⅢ-Ⅳand high malignancy in patients with poor prognosis were higher than those of the patients with good prognosis(P<0.05).Logistic multiple regression analysis showed that Ann Arbor stageⅢ-Ⅳ,moderate malignant,high malignancy,CASP8 rs 1035142 GT genotype,CASP8 rs 1035142 TT genotype and Fas rs 1377 GG genotype were all the risk factors for the poor prognosis of the patients(P<0.05).Conclusion:The poor prognosis rate of non-Hodgkin’s lymphoma patients in Han nationality is relatively high,and the risk factors for the prognosis of the patients include Ann Arbor stageⅢ-Ⅳ,moderate and high malignancy,CA SP8 rs 1035142 GT genotype,CASP8 rs 1035142 TT genotype and Fas rs 1377 GG genotype.