lncRNA RAB30-AS1对宫颈癌Hela细胞增殖和凋亡影响的机制研究

Effect of lncRNA RAB30-AS1 on the proliferation and apoptosis of cervical cancer Hela cells and its mechanism

目的探讨长链非编码RNA(lncRNA)RAB30-AS1对宫颈癌Hela细胞增殖和凋亡的影响。方法体外培养宫颈癌Hela细胞,分别转染pcDNA-NC(NC组)和pcDNA-RAB30-AS1(RAB30-AS1组),采用荧光定量PCR(qRT-PCR)检测转染效率。通过Edu试验和流式细胞术检测细胞增殖能力和凋亡能力的改变。qRT-PCR和蛋白质印迹法(Westernblot)检测磷脂酰肌醇蛋白聚糖-5(GPC5)基因的表达。结果NC组和RAB30-AS1组Hela细胞中RAB30-AS1的表达分别为(1.34±0.27)和(8.90±1.60),NC组RAB30-AS1表达明显低于RAB30-AS1组(P<0.01)。NC组和RAB30-AS1组Hela细胞增殖率分别为(41.82±2.86)%和(20.85±3.82)%,RAB30-AS1组细胞增殖率明显低于NC组(P<0.01)。NC组和RAB30-AS1组Hela细胞凋亡率分别为(12.61±1.96)%和(32.19±4.29)%,RAB30-AS1组细胞凋亡率明显高于NC组(P<0.01)。与NC组相比,RAB30-AS1组Hela细胞中GPC5基因表达显著增加(P<0.01)。结论RAB30-AS1过表达能够抑制宫颈癌Hela细胞的增殖、诱导细胞凋亡,其机制可能与促进GPC5基因表达有关。

Objective To explore the effect of long non-coding RNA(lncRNA)RAB30-AS1 on the proliferation and apoptosis of cervical cancer Hela cells.Methods Cervical cancer Hela cells were cultured in vitro,and transfected with pcDNA-NC(NC group)and pcDNA-RAB30-AS1(RAB30-AS1 group).qRT-PCR was used to detect the transfection efficiency.Edu experiment and flow cytometry were used to detect changes in cell proliferation and apoptosis.qRT-PCR and Western blot were used to detect the expression of glypican-5(GPC5)gene.Results The expressions of RAB30-AS1 of Hela cells in the NC group and RAB30-AS1 group were(1.34±0.27)and(8.90±1.60),respectively,and the expression of RAB30-AS1 in the NC group was significantly lower than that in the RAB30-AS1 group(P<0.01).The proliferation rates of Hela cells in the NC group and RAB30-AS1 group were(41.82±2.86)% and(20.85±3.82)%,respectively,and the proliferation rate of Hela cells in the RAB30-AS1 group was significantly lower than that in the NC group(P<0.01).The apoptosis rates of Hela cells in the NC group and RAB30-AS1 group were(12.61±1.96)% and(32.19±4.29)%,respectively,and the apoptosis rate of Hela cells in the RAB30-AS1 group was significantly higher than that in the NC group(P<0.01).Compared with that in the NC group,the expression of GPC5 gene in the RAB30-AS1 group significantly increased(P<0.01).Conclusions RAB30-AS1 overexpression can inhibit the proliferation of cervical cancer Hela cells and induce cell apoptosis,and its mechanism may be related to the promotion of GPC5 gene expression.