云南省2株柯萨奇病毒A12型VP1区基因特征分析

Genetic characteristics of VP1 region of two Coxsackievirus A12 strains in Yunnan province

目的:了解云南省手足口病(hand,foot and mouth disease,HFMD)监测中分离到的2株柯萨奇病毒A12型(Coxsackievirus A12,CV-A12)VP1区基因序列特征。方法:对HFMD监测中获得的临床标本开展RD细胞上的病毒分离培养,通过荧光定量PCR和测序方法鉴定为CV-A12的毒株,进一步对其VP1区进行序列测定,并搜集GenBank中CV-A12其他型别的参考株,共同构建系统进化树,分析CV-A12的VP1区基因特征。结果:获得2株CV-A12分离株的VP1区序列(888 bp),与2018年的云南参考株毒株同源性最高,其VP1区氨基酸在多个位点上与其他云南参考株存在特异突变。结论:云南省HFMD标本中发现的CV-A12已经发生地区特异性VP1基因变异。

Objective To do genetic analysis on the VP1 gene of 2 Coxsackievirus A12(CV-A12)isolated from hand,foot and mouth disease(HFMD)surveillance in Yunnan province.Methods Coxsackievirus isolation was carried out in RD cells from the clinical samples.CV-A12 strains were identified by realtime RT-PCR and sequencing technology from the positive cultures.The VP1 region of the CV-A12 strain was further sequenced.The VP1 gene sequences were initially aligned and then used to construct the phylogenetic tree with GenBank reference strains.Results The two CV-A12 strains had the highest homology with the Yunnan reference strains in 2018 and the amino acids in VP1 region had specific mutations with other cluster reference strains at multiple sites.Conclusions The CV-A12 in the HFMD cases in Yunnan province has occured regional specific mutation in VP1 gene.