摘要
在Balb/c小鼠模型上,评价双佐剂新型冠状病毒灭活疫苗的免疫效果及中和抗体水平.新型冠状病毒经Vero细胞培养、收获、浓缩、灭活、纯化等一系列步骤,制成灭活疫苗原液,以不同的配方剂量添加CpG佐剂和氢氧化铝佐剂后,免疫Balb/C小鼠,然后检测小鼠血清特异性抗体水平及中和抗体效价水平.结果表明:低、中剂量新冠抗原添加低、中剂量的CpG佐剂后,可以有效地提高S蛋白特异性抗体效价水平及中和抗体效价水平,效果远大于单独铝佐剂组;但CpG剂量如果过高,会有抑制效果.添加CpG佐剂后,可以使新冠病毒灭活抗原在较低的剂量下,就能产生较高水平的S蛋白特异性抗体效价及中和抗体效价.
This article valuates the immune effect and neutralization titer of the inactivated SARS-CoV-2 vaccine with dual adjuvant on the Balb/C mouse model.An inactivated vaccine bulk was prepared through a series of steps,which include culturing coronavirus in VERO cells,harvesting,concentrating,inactivating,and purifing.Then,CpG adjuvant and aluminum hydroxide adjuvant of different dosages were added and the Balb/C mouse was immunized.Finally,the level of specific antibody and neutralizing antibody in the serum of the mouse was detected.The results show that low and medium doses of inactivated SARS-CoV-2 antigens with addition of low and medium doses of CpG adjuvants can increase the titers of S protein specific antibodies and neutralizing antibodies effectively,andthe effect is much greater than that of the single aluminum adjuvant group;but if the CpG dose is too high,there will be an inhibitory effect.The inactivated SARS-CoV-2 antigen can produce a higher level of S protein specific antibody titers and neutralizing antibody titers at a lower dose after adding CpG adjuvant.
作者
王瑞博
许定花
刘家胜
张弓
柯江伟
龚昱斌
李方仲
韩璐
徐国华
司小伟
沈吉友
邵聪文
WANG Ruibo;XU Dinghua;LIU Jiasheng;ZHANG Gong;KE Jiangwei;GONG Yubin;LI Fangzhong;HAN Lu;XU Guohua;SI Xiaowei;SHEN Jiyou;SHAO Congwen(Zhejiang Toyouvax Bio-Pharmaceutical Co.,Ltd.,Hangzhou 311100,China)
出处
《昆明理工大学学报(自然科学版)》
北大核心
2021年第5期110-116,共7页
Journal of Kunming University of Science and Technology(Natural Science)
基金
杭州市余杭区新型冠状病毒感染的肺炎防治技术研究项目(2020YK005)。