膜联蛋白A7低表达对人胃癌MGC-803细胞迁移和侵袭的影响

Effects of Low Expression of ANXA7 on Migration and Invasion of Human Gastric Cancer MGC-803 Cells

目的探讨膜联蛋白A7(ANXA7)低表达对人胃癌MGC-803细胞迁移和侵袭的影响。方法培养人胃癌MGC-803细胞并分成3组,采用脂质体转染法,将靶向ANXA7的siRNA转染于干扰组细胞,阴性siRNA转染阴性对照组,空白对照组常规培养。转染48h后应用Western blotting检测各组ANXA7的表达以鉴定转染效率;细胞划痕实验检测各组细胞的迁移能力;Transwell实验检测细胞的侵袭能力;Western blotting检测ANXA7低表达后E-钙黏蛋白(E-cadherin)和基质金属蛋白酶(MMP-9)表达水平的变化。结果靶向ANXA7的siRNA显著抑制其在MGC-803细胞中的表达(P<0.05);干扰组细胞的迁移及侵袭能力均明显低于阴性组和空白组(P<0.05);E-cadherin的表达显著上调(P<0.05),MMP-9的表达显著下调(P<0.05)。结论ANXA7低表达可能通过调控E-cadherin和MMP-9的表达来抑制MGC-803细胞的迁移和侵袭能力。

Objective To investigate the effects of low expression of Annexin A7(ANXA7)on migration and invasion of human gastric cancer cells MGC-803.Methods Human gastric cancer MGC-803 cells were cultured and divided into 3 groups.Liposome transfection was used to transfect the targeted ANXA7 siRNA into the interference group cells,negative control siRNA was transfected into the negative control group,and the blank control group did not deal with any treatment.Cells from each group were tested for ANXA7 interference effi ciency by Western blotting forty eight hours after transfection.Then the migration and invasion abilities of MGC-803 cells were tested by Wound healing assay and Transwell assay.The expression levels of E-cadherin and MMP-9 were determined by Western blotting.Results siRNA targeting ANXA7 signifi cantly inhibited its expression in MGC-803 cell(s P<0.05);The migration and invasion ability of the interference group were signifi cantly lower than that of the negative group and the blank group(P<0.05);The expression of E-cadherin was signifi cantly up-regulated(P<0.05),mMP-9 expression was signifi cantly down-regulated(P<0.05).Conclusion The low expression of ANXA7 may inhibit the migration and invasion of MGC-803 cells by regulating the expression of E-cadherin and MMP-9.